In vitro intracellular staining of CD4+cells isolated from PFMC (N=10): The CD4+T cells were isolated from PFMC cultured with and without mycobacterial antigens (PPD, CF, MTB) by positive selection using anti-CD4 conjugated magnetic microbeads and purified using MACS column and dually stained for IFN-ã and IL-4. The figure represents the IFN-ã and IL-4 expression profile of CD4+T cells. Results represent the Mean ± SEM values. *Represents comparison of IFN-ã positive cells between control and PPD (P=0.04), control and CF (P=0.05), control and MTB (P=0.02). @Represents comparison of IL- 4 positive cells between control and PPD (P=0.03).