Indian J Med Microbiol Close
 

In vitro intracellular staining of CD4+cells isolated from PFMC (N=10): The CD4+T cells were isolated from PFMC cultured with and without mycobacterial antigens (PPD, CF, MTB) by positive selection using anti-CD4 conjugated magnetic microbeads and purified using MACS column and dually stained for IFN- and IL-4. The figure represents the IFN- and IL-4 expression profile of CD4+T cells. Results represent the Mean SEM values. *Represents comparison of IFN- positive cells between control and PPD (P=0.04), control and CF (P=0.05), control and MTB (P=0.02). @Represents comparison of IL- 4 positive cells between control and PPD (P=0.03).

In vitro intracellular staining of CD4+cells isolated from
PFMC (N=10): The CD4+T cells were isolated from PFMC cultured
with and without mycobacterial antigens (PPD, CF, MTB) by positive
selection using anti-CD4 conjugated magnetic microbeads and purified
using MACS column and dually stained for IFN- and IL-4. The figure
represents the IFN- and IL-4 expression profile of CD4+T cells. Results
represent the Mean  SEM values. *Represents comparison of IFN-
positive cells between control and PPD (P=0.04), control and CF
(P=0.05), control and MTB (P=0.02). @Represents comparison of IL-
4 positive cells between control and PPD (P=0.03).