Indian J Med Microbiol Close
 

Ex vivo intracellular staining of CD4+cells isolated from PFMC (N=10): The CD4+T cells were isolated from fresh PFMC by positive selection using anti-CD4 conjugated magnetic microbeads and purified using MACS column. These cells were then dually stained for IFN- and IL-4. The figure represents the IFN- and IL-4 expression profile of CD4+T cells. Results represent the Mean SEM values and *represents comparison between IFN- with IFN- + IL-4 (P=0.04), **represents comparison between IFN- with IL-4 (P=0.03).

Ex vivo intracellular staining of CD4+cells isolated from
PFMC (N=10): The CD4+T cells were isolated from fresh PFMC by
positive selection using anti-CD4 conjugated magnetic microbeads and
purified using MACS column. These cells were then dually stained for
IFN- and IL-4. The figure represents the IFN- and IL-4 expression
profile of CD4+T cells. Results represent the Mean  SEM values and
*represents comparison between IFN- with IFN- + IL-4 (P=0.04),
**represents comparison between IFN- with IL-4 (P=0.03).