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Intracellular cytokine: Analysis by FACS A representative FACS analysis of intracellular cytokine staining: The CD4+T cells were isolated from PFMC cultured with and without mycobacterial antigens (PPD, CF, MTB) by positive selection using anti-CD4 conjugated magnetic microbeads and purified using MACS column and dually stained for IFN- and IL-4. A total of 25000 events were acquired for each condition and quadrant analysis was done on Cell Quest software.

Intracellular cytokine: Analysis by FACS
A representative FACS analysis of intracellular cytokine staining: The
CD4+T cells were isolated from PFMC cultured with and without
mycobacterial antigens (PPD, CF, MTB) by positive selection using
anti-CD4 conjugated magnetic microbeads and purified using MACS
column and dually stained for IFN- and IL-4. A total of 25000 events
were acquired for each condition and quadrant analysis was done on
Cell Quest software.