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   1995| July-September  | Volume 13 | Issue 3  
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comparative studies on the susceptibility of certain primary cultures to coxiella burnetii
SS Kulkarni, RR Blonde, VS Ladybird
July-September 1995, 13(3):145-149
ABSTRACT: Ten primary cultures were screened to determine their susceptibility to Coxiella burnetii, the causative organism of Q fever. The most susceptible culture could then the be employed as an alternative system to the conventional yolk sac method for the efficient cultivation of these organisms. The primary cultures examined consisted of four each, derived from chick and mouse and two of human origin. The criteria followed for determining the susceptibility were microscopic counting of the number of infected cells, antigenicity and egg lethality assay. The maximum multiplication of C.burnetii was observed in the two human foetal cultures-human embryonic lung and kidney with cytopathic effect in the form of vacuolated cytoplasm. In the remaining cultures there was mild to moderate growth of C.burnetii. Our studies have generated sufficient data to show that, for the first time, certain primary cultures like human embryonic kidney, mouse embryonic brain, chorio-allantoic membrane and mouse bone marrow, are being reported as being susceptible to C.burnetii.
[ABSTRACT]   Full text not available   
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Finger printing of salmonella typhimurium : comparison between traditional and molecular methods
I Alikhan, A Ratan, T Fatima, F Gulkhan, A Kalia
July-September 1995, 13(3):141-144
ABSTRACT: Traditional methods for characterisation of Salmonella typhimurium failed to clonally resolve the isolates from an infant and powder milk as well as from three different epidemiologically unrelated patients. All the strains had same biochemical reactions, antibiogram, serotype and were nontypable by phage typing. Herein, we report the successful application of whole cell protein analysis (WCPA), by SDS-PAGE, and plasmid profile to establish the clonal nature of these isolates, WCPA and plasmid analysis yielded profiles that indicated towards a common source for the origin of the epidemiologically related isolates and could clonally resolve these from epidemiologically unrelated isolates.
[ABSTRACT]   Full text not available   
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Shingella Carriage in Children
M Saxena, S Aggarwal, V Gupta, S Sharma
July-September 1995, 13(3):131-133
ABSTRACT: This study was carried out to evaluate the carrier status of Shigella in school going children of 5-15 years of age in Chandigarh belonging to high, middle and low socio-economic status. In all, 3.5 percent (7/203) stool samples were positive for Shigella. No direct relationship with socio-economic status, age or sex was evident. Also contact tracing in selected families revealed absence of Shigella carriage in other members within the family. Further, to determine if the above isolates could act as foci of community infection, antibiotic resistance, Congo red binging and cell surface hydrophobicity of the isolates were done. All the isolates were found to be of low virulence.
[ABSTRACT]   Full text not available   
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characterization of cell wall associated polypeptides from different species of mycobacterium
AJ Mohamed, G Jayaraman
July-September 1995, 13(3):135-140
ABSTRACT: Cell wall associated proteins of various Mycobacterium species were isolated and characterized by SDS-PAGE and Western blotting to identify different strains/species of mycobacteria. Different species of mycobacterium were clearly distinguished, and only minor differences between two strains of Mycobacterium tuberculosis were obtained. Also, M.tuberculosis and M.bovis BCG were recognized as single species with high similarity. Western blot analysis revealed that some cell well associated proteins are major immunogenic antigens.
[ABSTRACT]   Full text not available   
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Occurrence of campylobacter jejuni/coli in cases of paediatric diarrhoea and guillain barre syndrome.
H Hariharan, C Kumaran, J Shanmugam, K Naseema, MD Nair, K Radhakrishnan, K Sabarinathan
July-September 1995, 13(3):125-129
ABSTRACT: One hundred cases of paediatric diarrhoea, and 8 cases of Guillain Barre Syndrome (GBS) from Kerala, were studied for the occurrence of Campylobacter jejuni, C. coli and C.lari by bacteriological cultures of faecal specimens. Thirteen percent of faecal swabs from children with diarrhoea were positive for campylobacters. Nine of 11 isolates identified to species level were C.jejuni, and the remaining were C.coli. Of eight GBS cases studied, 3(37.5 percent) were positive for C.jejuni/coli. All the 3 patients showed high titres of antibodies against a standard strain of C.jejuni, in a microplate agglutination test Antimicrobial drug susceptibility tests by disk diffusion assay on isolates from paediatric diarrhoea cases revealed strains resistant to ampicillin, erythromycin, gentamicin and/or tobramycin. All three isolates from the GBS cases showed multiple drug resistance: two isolates against drugs including ampicillin and erythromycin, and the third one against nalidixic acid and norfloxacin.
[ABSTRACT]   Full text not available   
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Unusual clinical presentations associated with filariasis
J Mahanta
July-September 1995, 13(3):155-157
ABSTRACT: Five cases of filariasis presented with unusual clinical manifestation are reported. One case with epididymal cyst showed adult filaria in it. In three cases microfilarae were demonstrated in the lesion as well as in the peripheral blood, who presented as cases of retroperitoneal cyst. lump in the abdomen, inta uterine foetal death (IUFD) and pericardial effusion respectively. One more case presented as angio neurotic oedema of lower lip, where filarial etiology was established by demonstrating microfilaria in the peripheral blood along with therapeutic response. All the cases were treated with Di-ethyl carbamazine citrate and followed up to four years. None of the cases did show any sign recurrence during the follow up period.
[ABSTRACT]   Full text not available   
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Protein and isoenzyme patterns of mycobacteria I. their role in identification of rapidly growing mycobacteria
VD Sharma, VM Katoch, CT Shivannavar, UD Gupta, RK Sharma, MS Patil, VP Bharadwaj, BM Agrawal
July-September 1995, 13(3):115-118
ABSTRACT: Cell free extracts (CFEs) from different rapidly growing mycobacteria namely, M.fortuitum, M.chelonei, M. vaccae, M.smegmatis, M.phlei and M.duvalii were electrophoresed and stained for proteins as well as lactate dehydrogenase (LDH), esterase, malate dehydrogenase (MDH), malic enzyme (ME), alanine dehydrogenase (Alanine DH), superoxide dismutase (SOD), alcohol dehydrogenase (Alcohol DH) and succinic dehydrogenase (SDH) activity. It was observed that combination of the protein, LDH, esterase and ME produced clearly distinguishable patterns for all the mycobacteria included in the study. Results described in the paper establish a simple electrophoretic technique for rapid identification of fast growing mycobacteria.
[ABSTRACT]   Full text not available   
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Protein and isoenzyme patterns of mycobacteria II, their role in identification of rapidly growing mycobacteria
VD Sharma, VM Katoch, CT Shivannavar, UD Gupta, RK Sharma, MA Patil, VP Bharadwaj, BH Agrawal
July-September 1995, 13(3):119-123
ABSTRACT: Cell free extracts (CFEs) from several slow growing mycobacteria namely m.asiaticum, M.avium, M.gastri, M.gordonae, M.intracellulare, M.kansasii, M.microti, M.nonchromogenicum, M.scrofulaceum, M.simiae, M.szulguai, M.triviale, M.tuberculosis, M.bovis BCG and M.xenopi were electrophoreses and stained for protein as well as lactate dehydrogenase (LDH), esterase, malate dehydrogenase (MDH), malic enzyme ME), alanine dehydrogenase (Alanine DH), and superoxide dismutase (SOD), alcohol dehydrogenase (Alcohol DH), and succinic dehydrogenase activity. It was observed that a combination of the protein and LDH produced clearly distinguishable patterns for all the mycobacteria included in the study. MDH and ME patterns were identified as additional useful taxonomic markers for identification of various slow growing nonchromogens and photochromogens. Identification could be completed within one working day after obtaining growth of these mycobacteria.
[ABSTRACT]   Full text not available   
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Eight year bacteriological study of gas gangrene in Pondicherry
RS Rao, MK Natarjan, I Ramesh
July-September 1995, 13(3):151-154
ABSTRACT: A total of 169 cases of gas gangrene were studied to identify the aetiological agents. Both aerobic and anaerobic organisms were isolated with a mean bacterial load of 4.05. clostridial group were isolated in 71 followed by anaerobic cocci in 71 and Bacteroides species in 54 cases. Clostridium perfringens was the most common isolated (30 cases). C.tetani was isolated in 18 cases. Aerobes like members of Enterobacteriaceae, Staphylococcus aureus, Beta-haemolytic Streptococci and Aeromonas species were isolated in 189,19,18 and 21 cases respectively. Since polymicrobial flora is commonly isolated in these cases it becomes imperative to know the aetiological agents to institute specific therapy to reduce the morbidity and mortality.
[ABSTRACT]   Full text not available   
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2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

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