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   1992| July-September  | Volume 10 | Issue 3  
    Online since November 3, 2009

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Prevalence Of R - Plasmids In Salmonella Typhimurium - An In-Vitro Study
Mamatha Shetty, Indira Bairy, P.G Shivananda
July-September 1992, 10(3):181-184
A total of 70 strains of salmonella typhimurium isolated from children with diarrhoeal disease were studied for the presence of R factors and their transfer to Escherichia coli K 12 F Nalidixic acid resistant. Of these 70 strains, one strain was resistant to single drug, 4 resistant to two drugs and remaining 64 strains were resistant to 3 or more drugs. All these strains were subjected to conjugative transfer study. Enbloc and partial transfer was seen in 10 (22.20%) and 35 (50.00%) respectively. Enbloc and partial secondary transfer was seen in 19 (48.70%) and 20 (51.30%) respectively in 45 transconjugants.
[ABSTRACT]   Full text not available  [PDF]
  824 40 -
Bacteriology Of Non-Specific Vaginitis With Special Reference To Gardnerella Vaginalis
Sarika Duggal, Satya Arora, Neerja Jindal, Rajiv Arora
July-September 1992, 10(3):185-188
Vaginal Swabs from 200 patients of nonspecific vaginitis (NSV) and 50 age matched controls were investigated Gardenerella Vaginalis was isolated in 36.5 cases of NSV and 8% controls. Quantitatively the growth was 3+or4+ on HBT medium in NSV and 1 + or 2 + In control group. Other aerobic organisms isolated in NSV epidermidis (11.5%). Amongst the controls the commonest aerobes were Staph albus and Esch. Coli 22% and 16% respectively. Isolated strains of G. Varinalis were most sensitive to Amplicillin (77.92%) followed by Kanamycin (54.55$) and metronidazole (44.16%).
[ABSTRACT]   Full text not available  [PDF]
  723 74 -
Strongyloides Stercoralis In Northern India
S Singh, M.P Sharma
July-September 1992, 10(3):197-203
From April 1988 to March 1989 an extensive search was carried out for the uncommon intestinal parasites like Strongyloides stercoralis and Trematodes in Northern India. Stool Samples were concentrated by the formal-ether centrifugation method and three cover slip mounts were made instead of one daily for 3-5 days. Following this method rhabtidiform larvae of Strongyloides, stercoralis were found in stool samples of 10 patients out of 2100 odd samples examined. Interestingly 7 out of these 10 patients had resided in Kumaun region of Uttar Pradesh from where Strongyloides, stercoralis has never been reported earlier. These patients were followed up and treated with Mebendazole but the drug was found ineffective and all patients required Thiabendazole. The clinical and demographic features of these patients are detailed.
[ABSTRACT]   Full text not available  [PDF]
  679 107 -
Anaerobic Bacterial Infection In Febrile Obstetrics Cases
R.C Pande, K.U Ansari, R Mehta, Krishna Mukherjee
July-September 1992, 10(3):207-210
Vaginal swabs of one hundred and eighty Obstetric cases of fever for more than 5 days duration were studied for anaerobic infection. Incidence of anaerobic positive cultures was found in 31.1 percent cases in pure cultures and in mixed culture with aerobic bacteria in 36.7 percent cases. Peptostreptococci were the commonest anaerobe isolated. The severity and incidence of clinical complications were significantly higher with anaerobic infections, as compared to mixed, aerobic & anaerobic infection and aerobic infections.
[ABSTRACT]   Full text not available  [PDF]
  600 68 -
Quality Assurance In Microbiology - A Guide For Developing Countries
J.J.S Snell, S.F Perry
July-September 1992, 10(3):143-163
In any assessment of the quality of microbiological laboratory services within a country, it is necessary to consider and evaluate many factors. This article attempts to describe a comprehensive range of activities that are components of total quality assurance. Some of these activities will not be appropriate to local circumstances and it is essential that each country develop their quality programme according to their individual needs and resources. For those starting on the path leading to improved quality, it can be profoundly discouraging to see the amount of work that needs to be done, often with inadequate resources. Although it may be useful to recognize what should be done in an ideal world, it must be recognized that the ideal may not be realizable in the short term, and perhaps never. This must not discourage laboratory workers and those responsible for the organization of laboratory services from attempting to improve quality. Many of the components of quality assurance are not unduly expensive and significant improvements can be achieved through alterations in the way that work is done and in people’s attitudes. The most significant need is to persuade people to think about quality; once this is achieved, the rest will follow.
[ABSTRACT]   Full text not available  [PDF]
  548 100 -
In Vitro Susceptibility Of Infecting Bacteria To Amoxycillin/Clavulanic Acid As Compared To Some Other Common Oral Antibiotics
S.C Sehgal
July-September 1992, 10(3):176-180
The in-vitro susceptibility of amoxycillin/clavulanic acid was determined against commonly isolated gram positive and gram-negative bacteria. This activity was independently compared with that of ampicillin, co-trimoxazole and cephalexin. The activity of amoxycillin/clavulanic acid was found to be much superior than the other antibiotics against many bacteria. This combination appears to be a better choice for the treatment of common infections especially for domiciliary use and initial therapy awaiting the bacteriology results.
[ABSTRACT]   Full text not available  [PDF]
  529 47 -
Comparison Of Mouse Pathogenicity Of Trichomonas Vaginalis From Symptomatic And Asymptomatic Cases
Renuka Bhat, Daksha Pandit, Lina Deodhar
July-September 1992, 10(3):189-192
Twelve isolates of Trichomonas recovered from symptomatic cases and 3 isolates recovered form asymptomatic cases were studied for their pathogenicity in mice using the subcutaneous route of inoculation. The average abscess size produced on the 7th day of inoculation by the strains recovered from symptomatic patients was 413 mm3 as compared to 141 mm3 produced by the strains from asymptomatic cases. The standard reference strain showed average abscess area of 358.5 mm3. Histopathological findings of the infected area correlated with the degree of infection in mice.
[ABSTRACT]   Full text not available  [PDF]
  537 31 -
Prevalence Of Delta Hepatitis In Coastal Karnataka
Jyothirlatha, Rama Ramani, Sabo George, P.G Shivananda
July-September 1992, 10(3):193-194
There is paucity of data from our country on the occurrence of HDV infection. Of the 48 serum samples positive for HBs Ag, 26 (54.2%) were positive for anti-HDV. The study showed high delta reactivity in chronic hepatitis cases (56.5%), in asymptomatic carriers it is (52%) and in acute hepatitis it is (50%).
[ABSTRACT]   Full text not available  [PDF]
  523 43 -
Comparison Of The Tube And The Micro-method For Identification Of Anaerobic Bacteria
Renu Bharadwaj, J.V Prakash, B.N Joshi, D.P Pandit
July-September 1992, 10(3):204-206
A miniaturized method for the identification of anaerobic bacteria is evaluated This employs a microtitre tray for substrate fermentation, aesculin hydrolysis, gelatin liquefaction and sport indole test. The results obtained are directly comparable with those produced by standard conventional methods in use at present..
[ABSTRACT]   Full text not available  [PDF]
  498 55 -
Usefulness Of Alternative Strategy For HIV Testing In India
S Kumari, Ramesh Aggarwal, D Chattopadhya
July-September 1992, 10(3):195-196
The conventional approach of HIV testing which relies on confirmation of all initial positive screening test using a Western Blot assay is expensive, time consuming and interpretation is difficult which leads to lot of indeterminate results. To reduce the number of Western blot assays, alternative strategy of using 2 ELISA combination yielded results which were comparable with conventional approach at a cost which is 1/7 the cost of using the conventional approach.
[ABSTRACT]   Full text not available  [PDF]
  475 31 -
Quality Control
R Sambasiva Rao
July-September 1992, 10(3):141-142
Full text not available  [PDF]
  299 131 -
Modulation Of Peripheral Blood Derived monocytes - Macrophages Of Leprosy Patients For Immunogenic Functions In-vitro By Tuftsin
Sangeeta Khare, Ravi R Iyer, D.N Rao
July-September 1992, 10(3):164-175
Full text not available  [PDF]
  351 38 -

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