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   1984| January-March  | Volume 2 | Issue 1  
    Online since January 16, 2010

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Molecular Studies On Drug Resistant Plasmid Of Salmonella Typhi : A Preliminary Report
P. L Sharma, K. B Sharma, K Prakash
January-March 1984, 2(1):57-61
Genetic characterization of S. typhi exhibited characteristic features of Inc H1 group plasmid such as thermosensitive transfer frequency and exclusion of F+ factor when introduced into K12F+ strain. Compatibility grouping study showed the presence of Inc H, group, R-plasmid in S. typhi. Molecular weight determination by agarose-gel electrophoresis revealed the presence of a single plasmid of 96 Md. (approx).
[ABSTRACT]   Full text not available   
  727 15 -
Intraoperative Wound Contamination During Abdominal Surgery
Arun Gupta, B.M.L Kapur
January-March 1984, 2(1):7-14
Ninety six patients undergoing laparotomy in a surgical unit were analysed for intra-operative wound containation. Cultures from the peritoneal cavity grew pathogenic contaminants in 37 patients (38.5%), and 26 patients (27.1%) were found to have pathogenic contaminants on the wound before skin stitches were applied. Staphylococcus aureus was the only pathogen cultured from 2 of the 21 clean wounds (9.5%). This contamination is probably preventable by a proper operating room conduct. Pathogenic contaminants were present in 24.6% of the contaminated and 64.3% of the dirty wounds. E. coli was the most common pathogen cultured. Suitable prophylactic antibiotics either locally on the wound or systemic or both, would continue to be required in these potentially contaminated procedures.
[ABSTRACT]   Full text not available   
  719 9 -
Enterotoxin Production By Various Salmonella Serotypes Isolated From Different Part Of India
Fouad Y Sobeh, K. B Sharma, K Prakash
January-March 1984, 2(1):67-72
Five hundred & twenty two strains of Salmonellae were isolated from cases of diarrhoea, septicemia and other sources during the years 1979-1983. Of the 522 strains, 46 were found sensitive to all the drugs tested. Prevalent antibiotic resistance pattern in Salmonellae were ACKST (18.6%), AKST (13.6%), ACKT (13.4%), CKT (10.2%) and ACGKT (9.4%). Enterotoxin production was demonstrated by gut ileal loop as well as rabbit’s skin permeability assay in 9.4% of the total strains. The most common toxigenic isolates were S. typhimurium (5.2%) and S. oranienburg (2.3%). Other serotypes viz; S. berta, S. chester, S. bareilly, S. enteritidis, S. Indiana, S. richmond and S. stanley were also among the enterotoxigenic strains. Both, resistant as well as sensitive strains of the various serotypes of Salmonella produced enterotoxin.
[ABSTRACT]   Full text not available   
  694 7 -
Essential Regions Of The Lipopolysaccharide Of Pseudomonas Aeruginosa Responsible For Ito Biological Activities
Yuzuru J Homma
January-March 1984, 2(1):1-6
Investigation of the relationship between the biological activities of the endotoxin of P. aeruginosa and chemical structure resulted in the dissociation of some of the biological activities of the endotoxin. Free lipid A was demonstrated to be responsible for the interferon-inducing activity, pyrogenicity and adjuvanticity. For manifesting inhibition of experimental tumor development or activation of proclotting enzyme of horseshoe crab (Tachypleus-tridentus), lipid A alone is not sufficient and a saccharide portion such as 3-deoxy-D-Manno-octulosonic acid is necessary to link the lipid A portion. That the polysaccharide portion plays a role in the activity of the complete lipopolysaccharide was also supported by the fact that chemical modification on the saccharide reduced the two activities drastically. Antitumor and interferon-inducing activities, adjuvanticity and activation of proclotting enzyme diminished by complete de-O-acylation by NH2 OH2 whereas interferon-inducing activity remained unchanged. Incomplete lipid A containing only amidelinked fatty acid is sufficient to induce interferon in vitro. None of the activities mentioned here was observed with degraded polysaccharide obtained after extraction of lipid A.
[ABSTRACT]   Full text not available   
  650 14 -
Study Of Bacillaemia In Leprosy
C. P Baveja, V. K Sharma, V. N Sehgal, D. S Agarwal
January-March 1984, 2(1):63-66
Using buffy-coat and haemolysis techniques, acid-fast bacilli (AFB) could be demonstrated in the blood stream of 10 out of 53 (18.9%) patients of leprosy. Of these 10 patients 9, belonged to lepromatous (LL) or borderline lepromatous (BL) spectrum and the remaining one was a borderline borderline (BB) case. None of the 30 patients towards tuberculoid (BT, TT) spectrum showed bacillaemia. A definite correlation was observed between the high bacterial index (BI) in the skin and detection of bacilli in the blood of leprosy patients. The buffy-coat technique proved superior to the haemolysis technique in demonstrating bacillaemia.
[ABSTRACT]   Full text not available   
  639 8 -
Evaluation Of Soluble Antigen Fluorescent Antibody (SAFA) Test In Laboratory Diagnosis Of Tuberculosis
Om P Bhardwaj, V. K Srivastava
January-March 1984, 2(1):39-48
Modified Soluble Antigen Fluorescent Antibody (SAFA) test was employed to find its utility in laboratory diagnosis of tuberculosis. A total number of 166 sera, 51 from patients of tuberculosis and remaining from control subjects were studied. Over eighty two percent of the 51 sera from patients of pulmonary tuberculosis were strong reactors; in addition 8.5% were weak reactors. Serologic reactivity was found unrelated to the extent or duration of disease. Out of 115 control subjects, only 2 were strong reactors; one of these was from a tuberculin positive child with pyrexia of unknown origin while the other was from a healthy blood donor who was lost to follow up. The high degree of specificity of the test seems to be attributable to use of Mycobacterial Saline Extract (M.S.E.) as the antigen and sensitivity to the test system used.
[ABSTRACT]   Full text not available   
  579 9 -
Heat Stress And Giardiasis
U Ganguly, P. C Sen
January-March 1984, 2(1):15-19
White albino mice (NMRI strain) were infected orally with seven strains of Giardia lamblia and the extent of infection was assessed from day 5 to day 21 by trophozoite count per cm. of small intestine. The trophozoite counts reached the maximum on day 11 and then started declining. Later mice were infected with seven more strains of Giardia lamblia and the infected animals were divided in two groups. One group of each strain were kept at 37oC and the other at 25oC to 25oC. The mean trophozoite counts in heat stressed animals were significantly higher on days 9, 11, 13, 15 and 18.
[ABSTRACT]   Full text not available   
  568 8 -
Lymphocyte Subpopulations And Their Functions In Quinine Administered Rhesus Monkeys
R. N Prasad, R. C Mahajan, N. K Ganguly, S. K Garg
January-March 1984, 2(1):25-31
The effect of quinine on lymphocyte subpopulations and their blastogenic responses have been studied in normal Rhesus monkeys. A decrease in percentage of lymphocyte sub-population and its functions was observed from the 3rd day following drug administration. Maximum decline was observed on 6th day when the concentration of quinine in plasma was 6.75+0.54 mg/litre, However, the effect of drug was undetectable by the 14th day when the level of drug was zero. Effect of quinine on lymphocytes and their functions seems to be dependent on time of exposure and concentration of drug.
[ABSTRACT]   Full text not available   
  566 5 -
Suppressive Effect Of Chloroquine On Humoral Response Of Normal Rhesus Monkeys
Ram Naresh Prasad, R. C Mahajan, N. K Ganguly, S. K Garg
January-March 1984, 2(1):21-24
Two groups of normal Rhesus monkeys were immunized with one ml of 50 percent suspension of sheep erythrocytes in normal saline of which one group was treated with chloroquine (20 mg/kg body weight) for 7 days. The directed plaque forming cells (PFC) was detected in peripheral blood of these animals. The number of PFC in chloroquine treated groups was significantly (P=001) lower than the control. It was concluded that the drug might act in autoimmune diseases by inhibiting the function of B-lymphocyte too. The inhibitory action of chloroquine on B cells may be beneficial during course of malaria infection by depressing the polyclonal activation of malarial mitogens.
[ABSTRACT]   Full text not available   
  558 6 -
Macrophage Migration Inhibition Test In Experimentally Giardia Lamblia Infected Mice
V Radhakrishna, N. K Ganguly, R. C Mahajan
January-March 1984, 2(1):73-78
Macrophage migration inhibition test was done with peritoneal macrophages obtained from the Giardia infected mice on different post infection days. There was no difference in the migration pattern of both the infected and control animals upto 9 the post infection day. A significant inhibition in migration as compared to the trophozoite counts was observed on the 12th and 15th post infection day. The effect of giardia infection on the delayed hypersensitivity as studied by macrophage migration inhibition index is discussed.
[ABSTRACT]   Full text not available   
  547 6 -
Development Of Cell-Mediated Immunity In Guinea Pigs Immunized With Ribonucleic Acid Protein Fraction Of Entamoeba Histolytica
T Sundararaj, A Sundararaj, S Subramanian
January-March 1984, 2(1):49-55
The development of cell-mediated immunity at various time intervals was studied after immunization of guinea pigs with ribonucleic acid protein fraction (P-RNA) obtained from Enamoeba histolytica. The parameters for the study of cell-mediated immunity were delayed skin hypersensitivity (DH), macrophage migration (MMI) and macrophage aggregation (MA) elicited with P-RNA protein and lysate antigens from E. histolytica. Delayed hypersensitivity developed 14 days after immunization and persisted upto 60 days and in a few animals upto 90 days. Macrophage migration inhibition response with P-RNA antigen was more marked after 30 days of immunization. The response seen with other antigens were not much marked. Macrophage aggregation was prominent when elicited with P-RNA antigen and corresponded with MMI response.
[ABSTRACT]   Full text not available   
  545 7 -
Immunological Studies In Cases Of Streptococcal Pyoderma
P. K Bhatnagar, K Prakash
January-March 1984, 2(1):33-38
Peripheral blood T and B lymphocytes, serum immunoglobulins, and complement (C3) levels were studied in 20 children with streptococcal pyoderma and compared with 20 normal controls. A significant depression in the proportion and absolute number of T cells was observed where as B cells were found to be significantly elevated in pyoderma cases. The levels of IgG were significantly higher than controls whereas the levels of IgM, IgA and C3 were not changed significantly when compared with the control group.
[ABSTRACT]   Full text not available   
  531 6 -

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