Indian Journal of Medical Microbiology Home 

ORIGINAL ARTICLE
[View FULLTEXT] [Download PDF]
Year : 2015  |  Volume : 33  |  Issue : 1  |  Page : 16--20

Tumour necrosis factor-alpha promoter polymorphism and its association with viral dilated cardiomyopathy in Indian population: A pilot study

B Mishra1, M Sharma1, S Sarkar1, A Bahl2, UN Saikia3, RK Ratho1 
1 Department of Virology, Postgraduate Institute of Medical Education and Research, Chandigarh, India
2 Department of Cardiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India
3 Department of Histopathology, Postgraduate Institute of Medical Education and Research, Chandigarh, India

Correspondence Address:
B Mishra
Department of Virology, Postgraduate Institute of Medical Education and Research, Chandigarh
India

Purpose: Tumour necrosis factor-α (TNF-α), a pro-inflammatory cytokine has been implicated in the pathophysiology of several viral infections. TNF-α promoter gene polymorphism is thus believed to play the modulating role in this disease pathogenesis. Several studies have shown the increased level of TNF-α in dilated cardiomyopathy (DCM). However, the role of the TNF-α promoter polymorphism is yet to be delineated in this regard. The present study for the first time tried to explore the association of TNF-α gene polymorphism with DCM of viral aetiology. Materials and Methods: Eighteen histopathologically proven DCM cases with viral genome positivity and 17 healthy controls were genotyped using polymerase chain reaction of TNF-α promoter gene followed by restriction fragment length polymorphism to determine the SNPs of -238G/A, -308G/A, -857C/T and -863C/A. Results: Of the 18 DCM cases 4 (22.2%) were positive for adenovirus (AdV), 2 (11.1%) for enterovirus (EV) and 12 (66.7%) had co-infection. Six of the 18 DCM cases (35.3%) had -238G/A polymorphism, and 10 (55.5%) had -863 homozygous AA genotype. The association of these polymorphisms was statistically significant as compared to controls (P < 0.05). Conclusions: The present pilot study suggests the possible association of TNFα -238G/A and -863C/A polymorphism with DCM of viral aetiology.

How to cite this article:
Mishra B, Sharma M, Sarkar S, Bahl A, Saikia U N, Ratho R K. Tumour necrosis factor-alpha promoter polymorphism and its association with viral dilated cardiomyopathy in Indian population: A pilot study.Indian J Med Microbiol 2015;33:16-20

How to cite this URL:
Mishra B, Sharma M, Sarkar S, Bahl A, Saikia U N, Ratho R K. Tumour necrosis factor-alpha promoter polymorphism and its association with viral dilated cardiomyopathy in Indian population: A pilot study. Indian J Med Microbiol [serial online] 2015 [cited 2020 Oct 30 ];33:16-20
Available from: https://www.ijmm.org/article.asp?issn=0255-0857;year=2015;volume=33;issue=1;spage=16;epage=20;aulast=Mishra;type=0