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Year : 2014  |  Volume : 32  |  Issue : 4  |  Page : 414--418

A possible alternative to the error prone modified Hodge test to correctly identify the carbapenemase producing Gram-negative bacteria

SS Jeremiah1, V Balaji2, S Anandan2, RD Sahni2 
1 Department of Microbiology, Velammal Medical College Hospital and Research Institute, Anuppanadi, Madurai, India
2 Department of Microbiology, Christian Medical College, Vellore, Tamil Nadu, India

Correspondence Address:
V Balaji
Department of Microbiology, Christian Medical College, Vellore, Tamil Nadu
India

Context: The modified Hodge test (MHT) is widely used as a screening test for the detection of carbapenemases in Gram-negative bacteria. This test has several pitfalls in terms of validity and interpretation. Also the test has a very low sensitivity in detecting the New Delhi metallo-β-lactamase (NDM). Considering the degree of dissemination of the NDM and the growing pandemic of carbapenem resistance, a more accurate alternative test is needed at the earliest. Aims: The study intends to compare the performance of the MHT with the commercially available Neo-Sensitabs - Carbapenemases/Metallo-β-Lactamase (MBL) Confirmative Identification pack to find out whether the latter could be an efficient alternative to the former. Settings and Design: A total of 105 isolates of Klebsiella pneumoniae resistant to imipenem and meropenem, collected prospectively over a period of 2 years were included in the study. Subjects and Methods: The study isolates were tested with the MHT, the Neo-Sensitabs - Carbapenemases/MBL Confirmative Identification pack and polymerase chain reaction (PCR) for detecting the blaNDM-1 gene. Results: Among the 105 isolates, the MHT identified 100 isolates as carbapenemase producers. In the five isolates negative for the MHT, four were found to produce MBLs by the Neo-Sensitabs. The Neo-Sensitabs did not have any false negatives when compared against the PCR. Conclusions: The MHT can give false negative results, which lead to failure in detecting the carbapenemase producers. Also considering the other pitfalls of the MHT, the Neo-Sensitabs - Carbapenemases/MBL Confirmative Identification pack could be a more efficient alternative for detection of carbapenemase production in Gram-negative bacteria.

How to cite this article:
Jeremiah S S, Balaji V, Anandan S, Sahni R D. A possible alternative to the error prone modified Hodge test to correctly identify the carbapenemase producing Gram-negative bacteria.Indian J Med Microbiol 2014;32:414-418

How to cite this URL:
Jeremiah S S, Balaji V, Anandan S, Sahni R D. A possible alternative to the error prone modified Hodge test to correctly identify the carbapenemase producing Gram-negative bacteria. Indian J Med Microbiol [serial online] 2014 [cited 2020 Nov 26 ];32:414-418
Available from: https://www.ijmm.org/article.asp?issn=0255-0857;year=2014;volume=32;issue=4;spage=414;epage=418;aulast=Jeremiah;type=0