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Year : 2012  |  Volume : 30  |  Issue : 4  |  Page : 403--406

Comparison of HIV-1 RNA level estimated with plasma and DBS samples: A pilot study from India (South)

S David, J Sachithanandham, J Jerobin, S Parasuram, R Kannangai 
 Department of Clinical Virology, Christian Medical College, Vellore, Tamil Nadu-632004, India

Correspondence Address:
R Kannangai
Department of Clinical Virology, Christian Medical College, Vellore, Tamil Nadu-632004
India

Purpose: The use of dried blood spots (DBS) for HIV-1 viral load determination could greatly enhance the management of HIV infected individuals in resource-limited countries. Objective: To compare the HIV-1 viral load values obtained between parallel collected plasma and DBS. Materials and Methods: DBS and plasma samples were collected from 62 HIV-1 infected individuals and were used for determination of HIV-1 RNA concentrations using the Abbot real-time HIV-1 PCR. Result: Mean of the log difference of viral load values between plasma and DBS was -0.41 log. DBS viral load values significantly correlated with plasma viral load (r = 0.9818, P < 0.0001). Conclusion: These results suggest that DBS samples can be used as an alternative to plasma for the estimation of HIV-1 viral load if samples are appropriately stored.

How to cite this article:
David S, Sachithanandham J, Jerobin J, Parasuram S, Kannangai R. Comparison of HIV-1 RNA level estimated with plasma and DBS samples: A pilot study from India (South).Indian J Med Microbiol 2012;30:403-406

How to cite this URL:
David S, Sachithanandham J, Jerobin J, Parasuram S, Kannangai R. Comparison of HIV-1 RNA level estimated with plasma and DBS samples: A pilot study from India (South). Indian J Med Microbiol [serial online] 2012 [cited 2020 Dec 1 ];30:403-406
Available from: https://www.ijmm.org/article.asp?issn=0255-0857;year=2012;volume=30;issue=4;spage=403;epage=406;aulast=David;type=0