Indian Journal of Medical Microbiology Home 

CORRESPONDENCE
[Download PDF]
Year : 2007  |  Volume : 25  |  Issue : 4  |  Page : 428--429

Microwave disinfection of gauze contaminated with bacteria and fungi

VH Cardoso1, DL Goncalves2, E Angioletto2, F Dal-Pizzol1, EL Streck1,  
1 Laboratory of Experimental Pathophysiology, University of the Far South Catarinense, 88806 . 000, Criciúma, SC, Brazil
2 Laboratory and Development of Antimicrobial Biomateriais and Materials, University of the Far South Catarinense, 88806 - 000, Criciúma, SC, Brazil

Correspondence Address:
E L Streck
Laboratory of Experimental Pathophysiology, University of the Far South Catarinense, 88806 . 000, Criciúma, SC
Brazil

How to cite this article:
Cardoso V H, Goncalves D L, Angioletto E, Dal-Pizzol F, Streck E L. Microwave disinfection of gauze contaminated with bacteria and fungi.Indian J Med Microbiol 2007;25:428-429

How to cite this URL:
Cardoso V H, Goncalves D L, Angioletto E, Dal-Pizzol F, Streck E L. Microwave disinfection of gauze contaminated with bacteria and fungi. Indian J Med Microbiol [serial online] 2007 [cited 2020 Oct 22 ];25:428-429
Available from: https://www.ijmm.org/text.asp?2007/25/4/428/37363

Full Text

Dear Editor,

Commercial radiation has been used to sterilize medical products for more than 40 years. [1],[2] Evidence from the literature clearly demonstrates that domestic microwave energy can be used for sterilization. [3],[4],[5] The equipment in routine use for the sterilization of surgical materials is moist heat sterilizer, i.e., autoclave. The main purpose for using microwave for sterilization is to save time. In this study, we verified whether microwave energy is able to disinfect gauze pieces colonized with Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923 or Candida albicans ATCC 10231 and compared this method to autoclave.

The microorganism concentration was adjusted to 0.5 in McFarland opacity scale. This solution was added to gauze pieces and submitted to microwave oven treatment at 1000 W from 10 to 60 seconds. Control samples were not submitted to this treatment. The gauze pieces were then inoculated in blood agar or brain heart infusion medium and incubated at 37 °C for 24 hours. Our results showed that exposure to microwave energy for 30 seconds was able to inhibit the growth of microorganisms [Figure 1]. When compared to humid heat sterilizer (temperature of 121 °C at 1.1 atmospheric pressure for a minimum of 20-30 minutes), similar results were found (data not shown).

In this work, we demonstrated that a domestic microwave oven could disinfect gauze pieces colonized with microorganisms. The material may be disinfected with exposure to microwave energy at 1000 W for 30 seconds.

References

1Morrissey RF, Herring CM. Radiation sterilization: Past, present and future. Radiat Phys Chem 2002; 63 :207-21.
2Hong SM, Park JK, Lee YO. Mechanisms of microwave irradiation involved in the destruction of fecal coliforms from biosolids. Water Res 2004; 38 :1615-25.
3Ikawa JY, Rossen JS. Reducing bacteria in household sponges. J Environ Health 1999; 62 :18-22.
4Silva MM, Vergani CE, Giampaolo ET, Neppelenbroek KH, Spolidorio DM, Machado AL. Effectiveness of microwave irradiation on the disinfection of complete dentures. Int J Prosthodont 2006; 19 :288-93.
5Campanha NH, Pavarina AC, Brunetti IL, Vergani CE, Machado AL, Spolidorio DM. Candida albicans inactivation and cell membrane integrity damage by microwave irradiation. Mycoses 2007; 50 :140-7.