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Year : 2007  |  Volume : 25  |  Issue : 3  |  Page : 267--271

The use of mtt [3-(4, 5-dimethyl-2-thiazolyl) -2, 5-diphenyl -2h- tetrazolium bromide]-reduction as an indicator of the effects of strain-specific, polyclonal rabbit antisera on Candida albicans and C. krusei

SN Arseculeratne1, DN Atapattu1, R Kumarasiri2, D Perera3, D Ekanayake3, J Rajapakse3 
1 Department of Microbiology, Faculty of Veterinary Sciences, University of Peradeniya, Sri Lanka
2 Department of Community Medicine, Faculty of Veterinary Sciences, University of Peradeniya, Sri Lanka
3 Faculty of Medicine and Veterinary Parasitology, Faculty of Veterinary Sciences, University of Peradeniya, Sri Lanka

Correspondence Address:
S N Arseculeratne
Department of Microbiology, Faculty of Veterinary Sciences, University of Peradeniya
Sri Lanka

There is only scanty data on the effects of specific antibody, with or without complement, on Candida albicans or Candida krusei in cell-free systems in vitro , although previously published work has shown that specific antibody mediates anti- Candida immunity in vivo by inhibition of adherence to host cells or surfaces and by the promotion of phagocytosis and intra-phagocytic killing. The MTT (3-[4, 5-dimethyl-2-thiazolyl] -2, 5-diphenyl -2H- tetrazolium bromide)-reduction method as a test of the viability of fungi was used to investigate the effect of complement, normal serum and immune serum on these two species of Candida that are of increasing importance as opportunistic pathogens. We report that normal rabbit serum or strain-specific, polyclonal anti- Candida rabbit antibody, with or without guinea pig complement, did not cause the reduction of total cell-mass or of the viability of either C. albicans or C. krusei, in vitro as determined by the MTT-reduction test. Complement alone without specific antibody, also, had no such effect on these two Candida species.

How to cite this article:
Arseculeratne S N, Atapattu D N, Kumarasiri R, Perera D, Ekanayake D, Rajapakse J. The use of mtt [3-(4, 5-dimethyl-2-thiazolyl) -2, 5-diphenyl -2h- tetrazolium bromide]-reduction as an indicator of the effects of strain-specific, polyclonal rabbit antisera on Candida albicans and C. krusei.Indian J Med Microbiol 2007;25:267-271

How to cite this URL:
Arseculeratne S N, Atapattu D N, Kumarasiri R, Perera D, Ekanayake D, Rajapakse J. The use of mtt [3-(4, 5-dimethyl-2-thiazolyl) -2, 5-diphenyl -2h- tetrazolium bromide]-reduction as an indicator of the effects of strain-specific, polyclonal rabbit antisera on Candida albicans and C. krusei. Indian J Med Microbiol [serial online] 2007 [cited 2021 Mar 8 ];25:267-271
Available from: https://www.ijmm.org/article.asp?issn=0255-0857;year=2007;volume=25;issue=3;spage=267;epage=271;aulast=Arseculeratne;type=0