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| Year : 1995 | Volume
: 13
| Issue : 3 | Page : 115--118 |
Protein and isoenzyme patterns of mycobacteria I. their role in identification of rapidly growing mycobacteria
VD Sharma, VM Katoch, CT Shivannavar, UD Gupta, RK Sharma, MS Patil, VP Bharadwaj, BM Agrawal
Dept of Microbiology, Central JALMA Institute for leprosy (ICMR), Taj Ganj, Agra
Correspondence Address:
VD Sharma
Dept of Microbiology, Central JALMA Institute for leprosy (ICMR), Taj Ganj, Agra
ABSTRACT: Cell free extracts (CFEs) from different rapidly growing mycobacteria namely, M.fortuitum, M.chelonei, M. vaccae, M.smegmatis, M.phlei and M.duvalii were electrophoresed and stained for proteins as well as lactate dehydrogenase (LDH), esterase, malate dehydrogenase (MDH), malic enzyme (ME), alanine dehydrogenase (Alanine DH), superoxide dismutase (SOD), alcohol dehydrogenase (Alcohol DH) and succinic dehydrogenase (SDH) activity. It was observed that combination of the protein, LDH, esterase and ME produced clearly distinguishable patterns for all the mycobacteria included in the study. Results described in the paper establish a simple electrophoretic technique for rapid identification of fast growing mycobacteria.
How to cite this article:
Sharma V, Katoch V, Shivannavar C, Gupta U, Sharma R, Patil M, Bharadwaj V, Agrawal B. Protein and isoenzyme patterns of mycobacteria I. their role in identification of rapidly growing mycobacteria.Indian J Med Microbiol 1995;13:115-118
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How to cite this URL:
Sharma V, Katoch V, Shivannavar C, Gupta U, Sharma R, Patil M, Bharadwaj V, Agrawal B. Protein and isoenzyme patterns of mycobacteria I. their role in identification of rapidly growing mycobacteria. Indian J Med Microbiol [serial online] 1995 [cited 2021 Mar 6 ];13:115-118
Available from: https://www.ijmm.org/article.asp?issn=0255-0857;year=1995;volume=13;issue=3;spage=115;epage=118;aulast=Sharma;type=0 |
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