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Year : 1993  |  Volume : 11  |  Issue : 3  |  Page : 170--177

Plasmid-Mediated Gentamicin Resistance In Methicillin Resistant Staph. Aureus In A Nursery Outbreak.

V Kaul 
 

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V Kaul


Gentamicin and methicillin resistant Staph. aureus (Gm - 76) was isolated from pus of a child with recurrent multiple abscesses. This strain spread to the nursery and infected eleven more patients. The Gm - 76 strain was investigated for the genetic determinant responsible for gentamicin resistance. Gentamicin resistant marker from Gm-76 strain was spontaneously lost which increased after treatment with ethidium bromide. This strain was lysogenic and mitomycin - C was effective phage inducer. A high frequency of transduction of gentamicin resistant marker was observed which decreased exponentially with UV - irradiated phage. An immediate phenotypic expression of gentamicin resistance was observed in transductants. As recombination deficient (rec-) strain was isolated after N-Methyl-N-nitro-N-nitrosoguanidine treatment form W 57 strains. The transduction frequency of the marker remained same when this strain was used as recipient. Gentmicin resistant plasmid was-isolated from gentamicin resistant parent strain. These results proved the plasmid nature of gentamicin resistance in Gm - 76 strain.

How to cite this article:
Kaul V. Plasmid-Mediated Gentamicin Resistance In Methicillin Resistant Staph. Aureus In A Nursery Outbreak. Indian J Med Microbiol 1993;11:170-177

How to cite this URL:
Kaul V. Plasmid-Mediated Gentamicin Resistance In Methicillin Resistant Staph. Aureus In A Nursery Outbreak. Indian J Med Microbiol [serial online] 1993 [cited 2021 Jan 16 ];11:170-177
Available from: https://www.ijmm.org/article.asp?issn=0255-0857;year=1993;volume=11;issue=3;spage=170;epage=177;aulast=Kaul;type=0