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Year : 2020  |  Volume : 38  |  Issue : 3  |  Page : 464-468

A real-time polymerase chain reaction-based approach for qualitative estimation of viral RNA in organ tissues of coxsackievirus A-16-infected neonatal mice


1 Enteric Viruses Group, ICMR-National Institute of Virology, Pune, Maharashtra, India
2 ICMR-National Institute of Virology, Mumbai Unit, Mumbai, Maharashtra, India

Correspondence Address:
Dr. Varanasi Gopalkrishna
ICMR-National Institute of Virology, 20-A, Dr. Ambedkar Road, Pune - 411 001, Maharashtra
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/ijmm.IJMM_20_117

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Hand, foot and mouth disease (HFMD) is a paediatric disease associated with enteroviruses (EVs). Among EVs, coxsackievirus A-16 (CVA-16) strain is currently in circulation and causing outbreaks in India. Neonatal mice (Institute of Cancer Research) strains were infected with CVA-16 strain isolated from HFMD patients to conduct pathological and molecular studies. Infected organs were harvested as per time points. A real-time polymerase chain reaction was used for qualitative estimation of viral RNA in organ tissues of infected mice. Skeletal muscle, brain tissue and cardiac tissues were the major target sites of CVA-16 tropism. The first-ever study was conducted on CVA-16 strains using the current approach in India.






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2004 - Indian Journal of Medical Microbiology
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