| ORIGINAL ARTICLE
|Year : 2020 | Volume
| Issue : 3 | Page : 401-408
Molecular diversity of dengue virus serotypes 1–4 during an outbreak of acute dengue virus infection in Theni, India
Amudhan Murugesan1, Dhanasezhian Aridoss1, Swarna Senthilkumar2, Lallitha Sivathanu2, Ramalingam Sekar2, Esaki M Shankar3, Elanchezhiyan Manickan4
1 Department of Microbiology, Government Theni Medical College, Theni; Department of Microbiology, Dr ALM PG IBMS, University of Madras, Chennai; Department of Microbiology, Virus Research and Diagnostic Laboratory, Government Theni Medical College, Theni, Tamil Nadu, India
2 Department of Microbiology; Department of Microbiology, Virus Research and Diagnostic Laboratory, Government Theni Medical College, Theni, Tamil Nadu, India
3 Infection Biology, Department of Life Sciences, School of Life Sciences, Central University of Tamil Nadu, Thiruvarur, Tamil Nadu, India
4 Department of Microbiology, Dr ALM PG IBMS, University of Madras, Chennai, Tamil Nadu, India
Background: Dengue fever (DF) is caused by an arthropod-borne dengue virus (DENV), has four serotypes and several genotypes. Although having clinical and epidemiological significance, the information on the circulating serotypes/genotypes is scarce in India.
Materials and Methods: Blood specimens were collected from the patients suspected of DF and they are tested for DENV NS1 antigen and DENV IgM by ELISA. Antigen-positive samples were further serotyped by reverse transcriptase polymerase chain reaction. Representative samples from each serotype were sequenced to identify the genotypes.
Results: All the four DENV serotypes were detected with the pre-dominance of DENV-1 (n = 49; 41.9%). Cases with multiple DENV serotype infections were also identified. Genotyping showed that DENV-1 belonging to genotype I, DENV-2 cosmopolitan (IV), DENV-3 genotype III and DENV-4 genotype I were active in the circulation during the outbreak in 2017.
Conclusion: Our study documents the molecular characteristics of DENV circulating in our geographical locality. The detection of heterologous DENV serotypes highlights the importance of regular molecular monitoring for the early recognition of any switch in pre-dominant serotype.
Dr. Amudhan Murugesan
Department of Microbiology, Government Theni Medical College, Theni, Tamil Nadu
Source of Support: None, Conflict of Interest: None
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