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 ORIGINAL ARTICLE
Year : 2019  |  Volume : 37  |  Issue : 3  |  Page : 363-369

Cross-country transport and isolation and identification of Streptococcus pneumoniae by use of alternate sources of blood supplemented media among laboratories in India


1 Medical Director, Manipal Clinics, Manipal Hospital, Bengaluru, Karnataka, India
2 Consultant Microbiologist, Manipal Hospital, Bengaluru, Karnataka, India
3 Consultant Microbiologist, Golwilkar Metropolis Health Services, (I) Pvt. Ltd., Pune, Maharashtra, India
4 Sr. Consultant, Department of Microbiology, BL Kapoor Memorial Hospital, New Delhi, India
5 Consultant Microbiologist, Shanti Mukund Hospital, New Delhi, India
6 Consultant Microbiologist, Yashomati Hospital, Bengaluru, Karnataka, India
7 M.S. Ramaiah Medical College, Bengaluru, Karnataka, India
8 Consultant Microbiologist, West Bank Hospital, Howrah, West Bengal, India
9 St. John's Medical College Hospital, Bengaluru, Karnataka, India
10 SS Microbiology Laboratory, Thane, Maharashtra, India
11 Consultant Microbiologist, Sri Aurobindo Seva Kendra, Kolkata, West Bengal, India
12 Consultant Microbiologist, Pushpanjali Hospital, New Delhi, India

Correspondence Address:
Dr. Satish Kumar Amarnath
Manipal Clinics, The Annexe, 98/1 Rustom Bagh, HAL (Old) Airport Road, Bengaluru - 560 017, Karnataka
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/ijmm.IJMM_19_82

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Background: The isolation of S. pneumoniae (Sp) depends on specimen integrity / transport, media and expertise. The non-availability of sheep blood agar poses a challenge in identification of colonial morphology and identification in India. Methods: Laboratories processed swabs containing either pure Sp or Sp in mixed cultures with a second (confounding) bacterium shipped across the country in cold conditions. Duplicate set of swabs was shipped back to the central laboratory to assess the impact of shipping on culture viability. The identical swab was cultured on sheep, human blood and one additional agar plate used in the laboratory. Results: 46/60(77%) of cultures containing only Sp were correctly identified. In specimens where Sp was present in mixed culture, the proportion of isolates in which Sp was correctly identified varied, with most variability attributed to the particular confounding organism rather than the media. There was no discernible impact of temperature-controlled (4-6°C) transport on the isolation of Sp from culture swabs. Conclusions: The study clearly elucidates the ability of laboratories for isolation of S. pneumoniae on human blood agar in resource limited settings. The results highlight the difficulties inherent in correctly identifying pathogens in mixed cultures in needs improvement using standardized tests across the study centers. The study also reaffirms the ability to transport biological specimens over long geographical distances without loss.






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2004 - Indian Journal of Medical Microbiology
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