An improvised medium for axenic cultivation of Acanthamoeba spp.

Kirti Megha; Amit Gupta; Rakesh Sehgal; Sumeeta Khurana

doi:10.4103/ijmm.IJMM_17_151

Indian Journal of Medical Microbiology

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Year : 2017 | Volume : 35 | Issue : 4 | Page : 597-599

An improvised medium for axenic cultivation of Acanthamoeba spp.

Kirti Megha¹, Amit Gupta², Rakesh Sehgal¹, Sumeeta Khurana¹
¹ Department of Medical Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh, India
² Department of Ophthalmology, Postgraduate Institute of Medical Education and Research, Chandigarh, India

Correspondence Address:
Dr. Sumeeta Khurana
Department of Medical Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh
India

Source of Support: None, Conflict of Interest: None

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DOI: 10.4103/ijmm.IJMM_17_151

Acanthamoebae can be easily grown in bacterised cultures, but their growth in axenic media is tedious and many times unsuccessful. We thus experimented with some additives in the conventional axenic medium for growth of various isolates of Acanthamoeba. Addition of Torula yeast RNA was found to significantly enhance the growth of Acanthamoebae in the axenic culture medium.

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