| BRIEF COMMUNICATION |
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| Year : 2016 | Volume
: 34
| Issue : 3 | Page : 350-352 |
Distribution of genes encoding aminoglycoside-modifying enzymes among clinical isolates of methicillin-resistant staphylococci
N Perumal, S Murugesan, P Krishnan
Department of Microbiology, Dr. ALM PG Institute of Basic Medical Sciences, University of Madras, Chennai, India
Correspondence Address:
P Krishnan
Department of Microbiology, Dr. ALM PG Institute of Basic Medical Sciences, University of Madras, Chennai
India
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/0255-0857.188339
The objective of this study was to determine the distribution of genes encoding aminoglycoside-modifying enzymes (AMEs) and staphylococcal cassette chromosome mec (SCCmec) elements among clinical isolates of methicillin-resistant staphylococci (MRS). Antibiotic susceptibility test was done using Kirby-Bauer disk diffusion method. The presence of SCCmec types and AME genes, namely, aac (6')-Ie-aph (2''), aph (3')-IIIa and ant (4')-Ia was determined using two different multiplex polymerase chain reaction. The most encountered AME genes were aac (6′)-Ie-aph (2'') (55.4%) followed by aph (3')-IIIa (32.3%) and ant (4')-Ia gene (9%). SCCmec type I (34%) was predominant in this study. In conclusion, the aac (6')-Ie-aph (2'') was the most common AME gene and SCCmec type I was most predominant among the MRS isolates.
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