|Year : 2016 | Volume
| Issue : 1 | Page : 109-110
Plasmid profile and antibiogram of Enterococcal faecalis isolated from tertiary care hospital in Delhi
M Barua, S Das, C Gupta, R Saha, IR Kaur
Department of Microbiology, UCMS and Guru Teg Bahadur Hospital, New Delhi, India
|Date of Submission||20-Jun-2014|
|Date of Acceptance||30-Apr-2015|
|Date of Web Publication||15-Jan-2016|
Department of Microbiology, UCMS and Guru Teg Bahadur Hospital, New Delhi
Source of Support: None, Conflict of Interest: None
|How to cite this article:|
Barua M, Das S, Gupta C, Saha R, Kaur I R. Plasmid profile and antibiogram of Enterococcal faecalis isolated from tertiary care hospital in Delhi. Indian J Med Microbiol 2016;34:109-10
|How to cite this URL:|
Barua M, Das S, Gupta C, Saha R, Kaur I R. Plasmid profile and antibiogram of Enterococcal faecalis isolated from tertiary care hospital in Delhi. Indian J Med Microbiol [serial online] 2016 [cited 2020 Oct 30];34:109-10. Available from: https://www.ijmm.org/text.asp?2016/34/1/109/174105
Multidrug resistant (MDR) enterococci are emerging as a leading nosocomial pathogen. Knowledge of antimicrobial resistance profile is essential to formulate treatment guidelines for infections caused by enterococci resistance (R) and plasmid biology is an important epidemiological tool to study resistance in enterococci. The study was conducted to look for endogenous plasmids in Enterococcal faecalis isolates circulating in a tertiary care hospital and analysed them for any correlation with their antibiogram pattern.
Over a period of 1-year, 40 E. faecalis isolated from clinical samples (blood, urine, wound swabs, urinary catheter tip, intravenous catheter tip, high vaginal swabs, endocervical swabs and peritoneal fluid) obtained from patients admitted to the hospital were tested for antibiotic sensitivity pattern as per Clinical and Laboratory Standards Institute guidelines  using Staphylococcus aureus ATCC 25923 and E. faecalis 29212 strains as standards. Plasmid DNA was extracted from all isolates using the mini preparation protocol method  and separated using horizontal gel electrophoresis system on 0.7% agarose gel along with Lambda Phage Hind III molecular weight marker – 46 kbp and 1 kbp DNA ladder used as the DNA marker. Plasmid DNA bands were visualised using ultraviolet transilluminator after staining with ethidium bromide. Statistical analysis was performed to determine typeability, reproducibility and discriminatory index of plasmid typing.
The plasmid was extracted in 39 isolates which were found to be MDR-resistance to more than three drugs  [Table 1], while one isolate was sensitive to all antibiotics and did not yield any plasmid. Four different plasmid patterns were observed (P1-4) with band pattern in different isolates varying from 1 to 4 and molecular weight ranging from >23 kbp to 564 kbp [Figure 1]. With a panel of 14 antibiotics, 4 different resistance patterns (R1-4) were observed; R2 was most commonly observed pattern [Table 1]. Plasmid typing has 100% typeability and reproducibility and the discriminatory index was calculated as 0.57.
|Table 1: Antibiotic susceptibility profile, resistotype distribution and plasmid profile of Enterococcus faecalis |
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|Figure 1: Plasmid profiles of various Enterococcus isolates. Lane M depicts molecular weight markers Lambda--Hind III. Lane 1–8 depicts plasmid profiles of Enterococcus faecalis isolates|
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Multidrug resistant strains with different resistant (R) pattern observed in this study suggest that there is common resistance gene pool existing in the population of Enterococcus species with high probability of free mobility of plasmid between the strains coding for resistance to several antibiotics; compatible with each other and are in circulation in the hospital. The plasmid typing can be utilised as a routine laboratory screening procedure for monitoring MDR bacterial strains. The study highlights the necessity of regular surveillance for all microorganisms encountered in hospital settings for monitoring the emerging resistance to antimicrobials.
| ~ Acknowledgment|| |
The authors are thankful to Dr. Bhudev C. Das, Former Director, Division of Molecular Oncology, Institute of Cytology and Preventive Oncology Noida for assisting in molecular work.
| ~ References|| |
Clinical and Laboratory Standards Institute (CLSI). Performance Standards for Antimicrobial Susceptibility Testing. CLSI Approved Standard. M-100-S16 (M7). Wayne, PA: Clinical and Laboratory Standards Institute; 2006.
Sambrook J, Russell DW. Molecular Cloning: A Laboratory Manual. 3rd
ed. New York: Cold Spring Harbor Laboratory Press; 2001.
Gagneja D, Goel N, Aggarwal R, Chaudhary U. Changing trend of antimicrobial resistance among gram-negative bacilli isolated from lower respiratory tract of ICU patients: A 5-year study. Indian J Crit Care Med 2011;15:164-7.