Indian Journal of Medical Microbiology IAMM  | About us |  Subscription |  e-Alerts  | Feedback |  Login   
  Print this page Email this page   Small font sizeDefault font sizeIncrease font size
 Home | Ahead of Print | Current Issue | Archives | Search | Instructions  
Users Online: 152 Official Publication of Indian Association of Medical Microbiologists 
  Search
 
  
 ~  Similar in PUBMED
 ~  Search Pubmed for
 ~  Search in Google Scholar for
 ~  Article in PDF (714 KB)
 ~  Citation Manager
 ~  Access Statistics
 ~  Reader Comments
 ~  Email Alert *
 ~  Add to My List *
* Registration required (free)  

 
 ~ Acknowledgement
 ~  References
 ~  Article Figures

 Article Access Statistics
    Viewed1778    
    Printed40    
    Emailed0    
    PDF Downloaded122    
    Comments [Add]    

Recommend this journal

 


 
  Table of Contents  
CORRESPONDENCE
Year : 2014  |  Volume : 32  |  Issue : 4  |  Page : 466-467
 

Impact of diet alteration on oral microflora by addition of probiotics


1 Department of Oral and Maxillofacial Pathology, Pacific Dental College and Hospital, Pacific Academy of Higher Education and Research Society University, Udaipur, Rajasthan, India
2 Department of Oral and Maxillofacial Pathology, Darshan Dental College and Hospital, Rajasthan University of Health Sciences, Udaipur, Rajasthan, India

Date of Submission15-Jul-2013
Date of Acceptance15-Jan-2014
Date of Web Publication4-Oct-2014

Correspondence Address:
M Astekar
Department of Oral and Maxillofacial Pathology, Pacific Dental College and Hospital, Pacific Academy of Higher Education and Research Society University, Udaipur, Rajasthan
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0255-0857.142242

Rights and Permissions



How to cite this article:
Astekar M, Sidhu G K, Kathuria N S. Impact of diet alteration on oral microflora by addition of probiotics . Indian J Med Microbiol 2014;32:466-7

How to cite this URL:
Astekar M, Sidhu G K, Kathuria N S. Impact of diet alteration on oral microflora by addition of probiotics . Indian J Med Microbiol [serial online] 2014 [cited 2020 Oct 26];32:466-7. Available from: https://www.ijmm.org/text.asp?2014/32/4/466/142242


Dear Editor,

The present study was conceded to access the influence of probiotics in the form of curd when given in the daily diet of the study group on the salivary levels of Streptococcus mutans and Lactobacilli at initial and after 3 months of follow up. Sample pool included 15 children in active group, who were given in their daily diet, and 15 children in control group, who were denied of food containing probiotics. All the children were followed for over a period of 3 months. A pre- and post-quantitative analysis of S. mutans and Lactobacilli were done in the saliva of both active and control groups. After incubation, the presence of S. mutans was confirmed by identifying light-blue to dark-blue raised colonies on the inoculated surface of the strip [Figure 1]a. The results were evaluated according to the manufacturer's guidelines as follows: Class 0: <10,000 CFU/ml, Class 1: <100,000 CFU/ml, Class 2: 100,000-1,000,000 CFU/ml, Class 3: >1,000,000 CFU/ml. The presence of Lactobacillus after incubation was evidenced by white to transparent colonies on the modified Rogosa agar surface [Figure 1]b. The results were evaluated according to the manufacturer's guidelines as Class 0: 10 3 CFU/ml, Class 1: 10 4 CFU/ml, Class 2: 10 5 CFU/ml, Class 3: 10 6 CFU/ml. Before taking the samples for both S. mutans and Lactobacilli, children were instructed to avoid eating, drinking and brushing of teeth for 1-2 h. During the 3 months follow-up, S. mutans decreased in 11 (73%) children (P = 0.165 not significant) and Lactobacillus increased in 10 (67%) (P = 0.000 significant) children in active group. There was no alteration seen in salivary S. mutans and Lactobacillus in control group. P - value was significant with P = 0.040 for S. mutans and P = 0.025 for Lactobacillus in control group. Similar results were found in a study carried by Ahola et al., after consumption of probiotic in the form of cheese over a period of 3 weeks. [1] Cagler et al., have also shown a reduced S. mutans level in oral cavity of patients receiving fluid or tablet in the form of probiotics. [2]
Figure 1: (a) Light-blue to dark-blue, raised colonies on the inoculated surface of the Streptococcus mutans strip. (b) White to transparent colonies of Lactobacillus on modified Rogosa agar surface

Click here to view



 ~ Acknowledgement Top


We would like to acknowledge Dr. Sonalee Shah, for her guidance and support.

 
 ~ References Top

1.Ahola AJ, Yli-Knuuttila H, Suomalainen T, Poussa T, Ahlstrom A, Meurman JH, et al. Short-term consumption of probiotic containing cheese and its effect on dental caries risk factors. Arch Oral Biol 2002;47:799-804.  Back to cited text no. 1
    
2.Cagler E, Kargula B, Tanboga I. Bacteriotherapy and probiotics' role on oral health. Oral Dis 2005;11:131-7.  Back to cited text no. 2
    


    Figures

  [Figure 1]



 

Top
Print this article  Email this article
 

    

2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04