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Year : 2014  |  Volume : 32  |  Issue : 4  |  Page : 419-422

Use of MALDI-TOF MS technique for rapid identification of bacteria from positive blood cultures

Department of Laboratory Medicine, Seoul National University Hospital, Seoul, Korea

Correspondence Address:
Eui-Chong Kim
Department of Laboratory Medicine, Seoul National University Hospital, Seoul
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0255-0857.142261

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We evaluated the feasibility of same-day routine aerobic bacterial identification using the following procedures: Picking colonies from 4 and 6 h incubated subculture from positive blood culture bottle and analyzing them by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS). The matched identification rate of this procedure at the species level was 80.6% (141/175) for the 4-h cultures compared with overnight cultures and 90.9% (159/175) for the 6-h cultures. Thus, our technique provides an easy and rapid method for identification of aerobic bacteria in routine clinical microbiology laboratories.


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2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04