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Year : 2014  |  Volume : 32  |  Issue : 3  |  Page : 331-332

Polymicrobial chronic endophthalmitis diagnosed by culture and molecular technique

1 Consultant Microbiologist, Sankara Nethralaya, Kolkata, West Bengal, India
2 Microbiologist, Sankara Nethralaya, Kolkata, West Bengal, India
3 Department of Microbiology, Sankara Nethralaya, Kolkata, West Bengal, India
4 Vitro-retina services, Consultant, Vitro-retina services, Sankara Nethralaya, Kolkata, West Bengal, India
5 Head of the Department, L&T Microbiology Research Centre, Sankara Nethralaya, Chennai, Tamil Nadu, India

Correspondence Address:
A Mukherjee
Consultant Microbiologist, Sankara Nethralaya, Kolkata, West Bengal
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0255-0857.136593

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Accurate etiological diagnosis is the key to prevention of ocular morbidity in endophthalmitis cases. A 66 year old male was suffering from chronic endophthalmitis post-cataract surgery. Polymerase chain reaction examination on anterior chamber fluid was positive for Propionibacterium acnes but negative for the panfungal genome. He was advised vitrectomy with intravitreal injections. Polymerase chain reaction of vitreous aspirate was positive for P.acnes as well as panfungal genome. The vitreous sample also grew yeast in culture which was identified as Candida pseudotropicalis. Patient was treated on an alternate day regimen of intravitreal Vancomycin and Amphotericin B in the post-operative period. There was improvement in vision at final follow up. Chronic endophthalmitis can have polymicrobial etiology which will require appropriate diagnostic and therapeutic strategies. The role of molecular testing is vital in these cases as growth in culture is often negative.


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2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04