Indian Journal of Medical Microbiology IAMM  | About us |  Subscription |  e-Alerts  | Feedback |  Login   
  Print this page Email this page   Small font sizeDefault font sizeIncrease font size
 Home | Ahead of Print | Current Issue | Archives | Search | Instructions  
Users Online: 1194 Official Publication of Indian Association of Medical Microbiologists 
 ~ Next article
 ~ Previous article 
 ~ Table of Contents
 ~  Similar in PUBMED
 ~  Search Pubmed for
 ~  Search in Google Scholar for
 ~  Article in PDF (35 KB)
 ~  Citation Manager
 ~  Access Statistics
 ~  Reader Comments
 ~  Email Alert *
 ~  Add to My List *
* Registration required (free)  

 ~  References

 Article Access Statistics
    PDF Downloaded143    
    Comments [Add]    

Recommend this journal


Year : 2006  |  Volume : 24  |  Issue : 4  |  Page : 303-304

Author's reply

Department of Infectious Diseases, Ranbaxy Research Laboratories, Gurgaon - 122001, Haryana, India

Date of Submission22-May-2006
Date of Acceptance26-May-2006

Correspondence Address:
Tarun Mathur
Department of Infectious Diseases, Ranbaxy Research Laboratories, Gurgaon - 122001, Haryana
Login to access the Email id

Source of Support: None, Conflict of Interest: None

Rights and PermissionsRights and Permissions

How to cite this article:
Mathur T. Author's reply. Indian J Med Microbiol 2006;24:303-4

How to cite this URL:
Mathur T. Author's reply. Indian J Med Microbiol [serial online] 2006 [cited 2020 Oct 26];24:303-4. Available from:

Dear Editor,

It was nice the views of Prof. P.K. Maiti about our paper.[1]

The objective of our study was to differentiate between biofilm producer and non-producer clinical strains of staphylococci by tissue culture plate (TCP) tube and Congo red agar methods described previously for detection of biofilm formation among staphylococci.

Staphylococcus epidermidis and Staphylococcus aureus are the most important pathogen in foreign body-related infections. The pathogenic mechanism by which they adhere to prosthetic devices and elaborate is now termed, as biofilm is increasingly understood. Identification of biofilm producing phenotype might help to elucidate the impact of staphylococci in diagnosis of infections related to biomedical devices.

These biofilm producing phenotype excrete an adherent cover glycocalyx or "slime", which allows 'planktonic" -unattached individual cells, to adhere to surfaces followed by cell-cell adhesion and accumulation of multilayered cell cluster surrounded by slimy cover termed as biofilms. This later part is mediated by polysaccharide intercellular adhesion (PIA), which is composed of partially N-acetylated linear β -1, 6-linked glucosaminoglycans and product of intracellular adhesion (ica) locus, icaADBC and one regulatory gene (icar).

Slime contribures the bulk of the volume of a biofilm and is primarily responsible for its slimy macroscopic properties. Detection of staphylococci with slimes is indicative of infected devices due to colonization by biofilm producing staphylococcal isolates.

In numerous in vitro studies related to adherence and biofilm formation by staphylococci was demonstrated on catheter disc, PVC tube, membranes, tissue culture plates, glass slide and glass beads, even when incubated for shorter incubation periods of 12-24 hours. These studies clearly indicated that biofilm formation is a continuous process and can be iniated as soon as the bacteria come in contact with foreign material. By both TCP and tube method only adherent cells of staphylococci covered with slime were deeply stained with crystal violet indicated the concentration of bacterial biofilms on the surface.

In our study, there were strain and media preparation variability of slime production in the presence of other carbohydrates. However, all non-slime producing staphylococcal strains were unable to produce slime either on polystyrene or glass material with similar media and incubation conditions. This fact correlates favourably with the methodology used by other investigators for detection of buiofilm producing phenotype among staphylococci.

Reference strains S. epidermidis ATCC 35984 (high slime producer) S. epidermidis ATCC 35983 (moderate slime producer) and S. epidermidis ATCC 12228 (non slime producer) included in our study behaved characteristically by all three methods.

 ~ References Top

1.Mathur T, Singhal S, Khan S, Upadhyay DJ, Fatma T, Rattan A. Detection of biofilm formation among the clinical isolates of Staphylococci: An evaluation of three different screening methods. Indian J Med Microbiol 2006; 24 :25-9.  Back to cited text no. 1    


Print this article  Email this article
Previous article Next article


2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04