Indian Journal of Medical Microbiology IAMM  | About us |  Subscription |  e-Alerts  | Feedback |  Login   
  Print this page Email this page   Small font sizeDefault font sizeIncrease font size
 Home | Ahead of Print | Current Issue | Archives | Search | Instructions  
Users Online: 1368 Official Publication of Indian Association of Medical Microbiologists 
 ~ Next article
 ~ Previous article 
 ~ Table of Contents
 ~  Similar in PUBMED
 ~  Search Pubmed for
 ~  Search in Google Scholar for
 ~Related articles
 ~  Article in PDF (105 KB)
 ~  Citation Manager
 ~  Access Statistics
 ~  Reader Comments
 ~  Email Alert *
 ~  Add to My List *
* Registration required (free)  

 ~  Abstract
 ~  Materials and Me...
 ~  Results
 ~  Discussion
 ~  References
 ~  Article Figures
 ~  Article Tables

 Article Access Statistics
    PDF Downloaded800    
    Comments [Add]    
    Cited by others 8    

Recommend this journal


Year : 2006  |  Volume : 24  |  Issue : 4  |  Page : 280-282

Serodiagnosis of dengue virus infection in patients presenting to a tertiary care hospital

1 Department of Microbiology, Nizamís Institute of Medical Sciences, Punjagutta, Hyderabad - 500 082, Andhra Pradesh, India
2 Department of Internal Medicine, Nizamís Institute of Medical Sciences, Punjagutta, Hyderabad - 500 082, Andhra Pradesh, India

Date of Submission21-Oct-2005
Date of Acceptance24-May-2006

Correspondence Address:
V Lakshmi
Department of Microbiology, Nizamís Institute of Medical Sciences, Punjagutta, Hyderabad - 500 082, Andhra Pradesh
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0255-0857.29387

Rights and Permissions

 ~ Abstract 

Dengue is an acute infectious disease of viral etiology. It is probably one of the most important arthropod borne viral disease in terms of human morbidity and mortality. The spectrum of disease ranges from self-limited dengue fever to more severe forms of dengue hemorrhagic fever (DHF) or dengue shock syndrome (DSS). Laboratory diagnosis of dengue virus infection mainly depends on detection of virus specific antibodies. The aim of the study was to correlate the serological results with clinical presentation in patients with a diagnosis of dengue. Eleven out of 15 (73.3%) patients with DHF and DSS had secondary antibody response and mortality was 100% in these patients.

Keywords: Dengue haemorrhagic fever, dengue shock syndrome, IgG, IgM, primary infection, secondary infection

How to cite this article:
Neeraja M, Lakshmi V, Teja V D, Umabala P, Subbalakshmi M V. Serodiagnosis of dengue virus infection in patients presenting to a tertiary care hospital. Indian J Med Microbiol 2006;24:280-2

How to cite this URL:
Neeraja M, Lakshmi V, Teja V D, Umabala P, Subbalakshmi M V. Serodiagnosis of dengue virus infection in patients presenting to a tertiary care hospital. Indian J Med Microbiol [serial online] 2006 [cited 2020 Dec 1];24:280-2. Available from:

Dengue, also called classic dengue or break bone fever, is a flaviviral infection found in large areas of tropical and subtropical regions.[1] Dengue is a mosquito borne viral infection and is transmitted by Aedes aegypti and Aedes albopictus.[2],[3] Four distinct serotypes of viruses DEN-1, DEN-2, DEN-3 and DEN-4[3],[4] cause dengue. Humans are the main-amplifying hosts of the virus.[1] Dengue may be asymptomatic or may lead to undifferentiated fever, dengue fever (DF) or dengue hemorrhagic fever (DHF) and dengue shock syndrome.[5] DHF and dengue shock syndrome (DSS) are leading causes of hospitalization and death especially among children.[3],[6] The environmental risk factors for DHF are infestations with Aedes mosquito, hot and humid climates enhancing mosquito breeding, mosquito density and presence of all the four serotypes of dengue virus with secondary infection in the host, the water storage pattern in the houses, population density and large movement of people towards urban areas.[1] Early diagnosis of dengue is important and can be established with commercially available serological assays.[1] Early case detection and management reduce morbidity and mortality due to DHF or DSS.

The main objective of this study was to evaluate the utility of serodiagnosis of dengue virus infection and correlate the results with demographic, clinical and laboratory profile in patients clinically diagnosed to be suffering from dengue at a tertiary care hospital in Hyderabad, Andhra Pradesh, South India.

 ~ Materials and Methods Top

During the period between September to December 2004, there was an outbreak of dengue in the city of Hyderabad and surrounding districts, in the state of Andhra Pradesh, South India. Two hundred and sixty patients suspected to be suffering from dengue fever /DHF/DSS were referred to our Institute. Serum collected from all the 260 patients were tested for IgG and IgM anti dengue antibodies by dengue IgG capture enzyme-linked immunosorbent assay (ELISA) and dengue IgM capture ELISA (PanBio Pty Ltd, Queensland, Australia). The dengue/DHF case investigation forms (WHO) were filled in by clinicians.[7] Case records of 100/211 seropositive patients who needed admission, were analyzed for demographic, clinical and laboratory data. The remaining 111 seropositive patients were managed on a conservative out-patient basis.

As per the WHO classification, the proposed probable diagnosis - an acute febrile illness with two or more of the following manifestations - headache, retro-orbital pain, arthralgia, rash, haemorrhagic manifestations, leucopenia and a positive IgM antibody test on serum samples collected five or more days after the onset of fever supports the diagnosis of dengue.[5] A primary infection is indicated when the IgM to IgG index value ratio is 1.78 and secondary infection is indicated when the IgM to IgG ratio is less than 1.78.[8] Taking these criteria into account, patients have been categorized as suffering from primary/secondary infections.

 ~ Results Top

Two hundred and eleven of the 260 suspected cases of dengue were seropositive and 100 of these seropositive patients required admission. The age of the seropositive patients ranged between 9 to 78 years. The most affected age group was between 20 to 39 years. The male to female ratio of the seropositive patients was 2:1 [Figure - 1]. Since, our Institute is situated at Hyderabad in the Telangana region of Andhra Pradesh, most of the patients included in this study were referred from the surrounding districts in this region. Majority of the patients were from the city of Hyderabad, followed by Karimnagar, Warangal and Khammam [Figure - 2].

The major clinical findings in the reported DF cases were febrile illness, thrombocytopaenia, severe headache, nausea and vomiting [Table - 1].

In the five patients who progressed to DHF, the major clinical findings were abrupt onset of high fever, capillary plasma leakage and bleeding tendencies [Table - 2].

Ten patients progressed to DSS. They had persistent hypotension, rapid and weak pulse, restlessness, cold and clammy skin. Of the 211 sera, which gave positive results with IgG/IgM capture ELISA, IgG antibodies only were detected in 40.28% cases, IgM antibodies only in 3.8% cases and both IgG and IgM in 55.9% cases. Secondary dengue infections were more predominant (79%) in our series. On correlating the serological results with the clinical types of presentation of dengue among the study patients, 68/85 (80%) DF patients and 11/15 (73.3%) patients with DHF or DSS had a secondary antibody response. While all the 85 patients with uncomplicated DF survived, 3/5 (60%) patients with DHF and 8/10 (80%) patients with DSS expired. In all 11 of the expired patients there was a secondary antibody response with 100% mortality [Figure - 3].

 ~ Discussion Top

The epidemiology of dengue in the Telangana region of Andhra Pradesh showed that most reported cases occurred in young adults. Our data shows that the epidemic waves were seen in urban centers, because highly urbanized areas have substantial proportions of the populations living in crowded impoverished areas with poor sanitation.[1] These complex urban settings are present in many regions in Hyderabad and surrounding districts posing a major challenge to vector control activities.

Among the clinical signs, we noticed a higher proportion of febrile syndrome, thrombocytopaenia, severe headache, nausea, vomiting, myalgia and rash in patients with DF. DHF is a biphasic febrile illness, with hemorrhagic tendencies due to plasma leakage and increased vascular permeability.[5]

Laboratory criteria for confirmation of dengue fever are isolation of dengue virus from serum and detection of dengue virus genomic sequences by polymerase chain reaction.[9] However, these tests are available only in reference laboratories and not available routinely. Hence, serological tests that demonstrate a fourfold or greater rise in reciprocal IgG or IgM antibody titre to one or more dengue virus antigens in the serum have greatly enhanced our ability to effectively and efficiently diagnose dengue infections.

The serological tests using IgM capture and IgG capture ELISA in which the cut off value of the IgG is set to discriminate between high levels of IgG (characteristic of secondary dengue infections) and lower IgG (characteristic of primary /past dengue).[10] With this method, a majority of secondary dengue infections could be clearly delineated. Quantitation of antidengue IgG in these tests allows distinction between primary and secondary infections. In this study, 153/211(72.5%) showed secondary serological response and was more predominant among all the types of dengue. High mortality in the DHF/DSS cases with secondary response indicates that emphasis is needed for a close monitoring and conservative management to reduce morbidity and mortality. In spite of best treatment given to DSS patients, they succumbed to the infection because of late presentation of these patients to the hospital. The DSS patients were already in shock during their presentation to the hospital and in spite of adequate fluids and supportive therapy given, they could not be revived. Among the patients who expired with DHF and DSS, secondary infections were observed, indicating the need for their conservative management to decrease the incidence of deaths. In our study, early serodiagnosis and admission of suspected dengue cases and prompt administration of intravenous fluids to individuals showing signs of premonitory vascular collapse have brought case fatality rates down sharply in dengue endemic areas.

We believe that serological diagnosis has a role in categorizing primary and secondary dengue and it also serves as a predictor of disease progression and mortality especially in the severe forms (DHF and DSS).

 ~ References Top

1.Chaturvedi UC, Shrivastava R. Dengue haemorrhagic fever: A global challenge. Indian J Med Microbiol 2004; 22 :5-6.  Back to cited text no. 1    
2.Tsai TF . Flaviviruses. Yellow fever. Dengue DH, JE. St.Louis encephalitis, Tick-borne encephalitis. Chapter 142. In : Principles and practice of infectious diseases , 5th ed. Mandell, Bennett, Dolin . Churchill Livingston: Pennsylvania, USA; 2000. p. 1716.   Back to cited text no. 2    
3.Lanciotti RS, Calisher CH, Gubler DJ, Chang GJ, Vorndam AV. Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction . J Clin Microbiol 1992; 30 :545-51.  Back to cited text no. 3    
4.Siqueira JB, Martelli CM, Coelho GE, Simplicio AC, Hatch DL. Dengue and dengue haemorrhagic fever, Brazil, 1981-2002 . Emerg Infect Dis 2005; 2 :48-53.  Back to cited text no. 4    
5.World Health Organization . Dengue haemorrhagic fever. Diagnosis, treatment, prevention and control. 2nd ed. Chapter 2. World Health Organization : Geneva, Switzerland; 1997.  Back to cited text no. 5    
6.Narayanan M, Aravind MA, Ambikapathy P, Prema R, Jeyapaul MP. Dengue fever: Clinical and laboratory parameters associated with complications. Dengue Bull 2003; 27 :108-15.  Back to cited text no. 6    
7.World Health Organization . World Health Organization. Dengue Bull : Geneva, Switzerland; 2003. p. 27.   Back to cited text no. 7    
8.Porter KR, Widjaja S, Lohita HD, Hadiwijaya SH, Maroef CN, Suharyono W, et al . Evaluation of a commercially available immunoglobulin M capture Enzyme-Linked Immunosorbent assay kit for diagnostic acute dengue infections . Clin Diagn LabImmunol 1999; 6 :741-4.  Back to cited text no. 8    
9.World Health Organization . Dengue haemorrhagic fever. Diagnosis, treatment, prevention and control. 2nd ed. Chapter 4. World Health Organization : Geneva, Switzerland; 1997.   Back to cited text no. 9    
10.Cuzzubbo AJ, Vaughn DW, Nisalak A, Solomon T, Kalayanarooj S, Aaskov J, et al . Comparison of PanBio dengue duo enzymelinked immunosorbent assay (ELISA) and MRL dengue fever virus immunoglobulin M capture ELISA for diagnosis of dengue virus infections in Southeast Asia. Clin Diagn Lab Immunol 1999; 6 :705-12.  Back to cited text no. 10    


[Figure - 1], [Figure - 2], [Figure - 3]


[Table - 1], [Table - 2]

This article has been cited by
1 Unusual and rare manifestations of dengue during a dengue outbreak in a tertiary care hospital in South India
M. Neeraja,V. lakshmi,V. D. Teja,Vanjari Lavanya,E. N. Priyanka,K. Subhada,M. M. Parida,P. K. Dash,Sashi Sharma,P. V. L. Rao,Gopal Reddy
Archives of Virology. 2014;
[Pubmed] | [DOI]
2 Epidemiological trends and clinical manifestations of Dengue among children in one of the English-speaking Caribbean countries
A. Kumar,M. Gittens-St Hilaire,A. L. Nielsen
Transactions of the Royal Society of Tropical Medicine and Hygiene. 2013; 107(4): 254
[Pubmed] | [DOI]
3 Frequency, Pattern and Management of Acute Abdomen in Dengue Fever in Karachi, Pakistan
Muhammad Shamim
Asian Journal of Surgery. 2010; 33(3): 107
[Pubmed] | [DOI]
4 Frequency, pattern and management of acute abdomen in dengue fever in Karachi, Pakistan
Shamim, M.
Asian Journal of Surgery. 2010; 33(3): 107-113
5 Subclinical cardiac involvement in dengue haemorrhagic fever
Gupta, V.K., Gadpayle, A.K.
Journal, Indian Academy of Clinical Medicine. 2010; 11(2): 107-111
6 Clinical and laboratory features that distinguish dengue from other febrile illnesses in endemic populations
Potts, J.A., Rothman, A.L.
Tropical Medicine and International Health. 2008; 13(11): 1328-1340
7 Haematological and biochemical indicators for the early diagnosis of dengue viral infection
Butt, N., Abbassi, A., Munir, S.M., Ahmad, S.M., Sheikh, Q.H.
Journal of the College of Physicians and Surgeons Pakistan. 2008; 18(5): 282-285
8 Clinical and laboratory features that distinguish dengue from other febrile illnesses in endemic populations
James A. Potts,Alan L. Rothman
Tropical Medicine & International Health. 2008; 13(11): 1328
[Pubmed] | [DOI]


Print this article  Email this article
Previous article Next article


© 2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04