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Year : 2006  |  Volume : 24  |  Issue : 3  |  Page : 201-204

Simple and economical method for speciation and resistotyping of clinically significant coagulase negative staphylococci

Department of Microbiology, University College of Medical Sciences and GTB Hospital, New Delhi - 110 095, India

Correspondence Address:
N P Singh
Department of Microbiology, University College of Medical Sciences and GTB Hospital, New Delhi - 110 095
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Source of Support: None, Conflict of Interest: None

PMID: 16912440

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An attempt was made to speciate 102 clinically significant isolates of coagulase negative staphylococci (CoNS) by a practical scheme adapted from various references. This scheme utilizes slide and tube coagulase test, urease test ornithine decarboxylase, novobiocin susceptibility and aerobic acid from mannose for assigning species group. Inclusion of one or two additional tests in a species group could identify the isolates to species level. Ninety eight (97%) isolates were conveniently identified as S. epidermidis (41%), S. saprophyticus (16.6%), S. haemolyticus (14.7%), S. hominis (14.7%), S. lugdunensis (4.9%), S. schleiferi (1.9%) and S. capitis (1.9%). Only four isolates were not identified to the species level, two of which were probably S. capitis subspecies ureolyticus / S. warneri / S. simulans . Antibiotic susceptibility testing showed maximum resistance to ampicillin (89%) followed by cefotaxime (59%) with no resistance to vancomycin. The increasing recognition of pathogenic potential of CoNS and emergence of drug resistance amongst them denotes the need to adopt simple laboratory procedures to identify and understand the diversity of staphylococci isolated from clinical material.


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2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

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