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Year : 2005  |  Volume : 23  |  Issue : 3  |  Page : 172-175

Non tuberculous mycobacteria isolated from clinical specimens at a tertiary care hospital in South India

Department of Clinical Microbiology , Christian Medical College, Vellore - 632 004, India

Date of Submission22-Feb-2005
Date of Acceptance27-Apr-2005

Correspondence Address:
M V Jesudason
Department of Clinical Microbiology , Christian Medical College, Vellore - 632 004
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0255-0857.16589

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 ~ Abstract 

Purpose: This is a retrospective analysis of the isolation rates of nontuberculous mycobacteria (NTM) from various clinical specimens and their antimicrobial susceptibility patterns. Methods: All NTM isolated between 1999 and 2004 at Christian Medical College, Vellore, South India, were identified with various biochemical tests. Antimicrobial susceptibility test for all NTM was performed by standard methods. Results : A total of 32,084 specimens were received for culture, of which 4473 (13.9%) grew acid fast bacilli (AFB). Four thousand three hundred (96.1%) of the AFB were M. tuberculosis while 173 (3.9%) were NTM. Of the 173 NTM, 115 (66.5%) were identified to the species level. Pus, biopsy specimens and sputum specimens yielded most of the NTM of which M. chelonae (46%) and M. fortuitum (41%) accounted for majority of them. M. chelonae and M. fortuitum , showed highest susceptibility to amikacin (99.2%). NTM were repeatedly isolated from seven sputum specimens, 15 biopsy and pus specimens, two CSF and two blood cultures. Six were isolated from patients with AIDS and five from post transplant patients. Conclusions: The isolation of NTM from various clinical specimens is reported in this study to highlight the associated diseases and therapeutic options in these infections.

Keywords: NTM, M. chelonae, M. fortuitum

How to cite this article:
Jesudason M V, Gladstone P. Non tuberculous mycobacteria isolated from clinical specimens at a tertiary care hospital in South India. Indian J Med Microbiol 2005;23:172-5

How to cite this URL:
Jesudason M V, Gladstone P. Non tuberculous mycobacteria isolated from clinical specimens at a tertiary care hospital in South India. Indian J Med Microbiol [serial online] 2005 [cited 2021 Jan 23];23:172-5. Available from:

Although M. tuberculosis, M. bovis and M. leprae are established pathogens predominating human mycobacterial infections, nontuberculous mycobacteria (NTM) are increasingly being reported as etiological agents of human infections.[1] NTM comprising of over 95 species are naturally seen as saprophytes[2] but are known to cause four different categories of infections in humans such as, i) pulmonary infections resembling tuberculosis, ii) extra pulmonary infections affecting lymph nodes, skin and soft tissue, iii) multifocal disseminated infections and iv) infections in immunocompromised individuals such as AIDS and transplant patients.[3] The growing population of HIV infected individuals and other immunosuppessed / immunocompromised patients coupled with better diagnostic techniques has led to an increase in the number of NTM being reported in human infections in recent years.[4]

NTM produce infections more commonly in the presence of predisposing factors / underlying diseases; they are also notably resistant to commonly used antitubercular drugs. These factors augment morbidity and limit therapeutic options in such infections. NTM have been reported world wide with varying frequencies, [5],[6],[7],[8] while in India isolation rates are between 0.7% and 34%.[9] We have reviewed the isolation rates of NTM from various clinical specimens over a period of six years at the department of Clinical Microbiology, Christian Medical College, Vellore - South India. Through this study we wish to emphasize the need to look for NTM in various clinical specimens.

 ~ Materials and Methods Top

This study includes the isolation of NTM from all clinical specimens from suspected pulmonary and extra pulmonary tuberculosis received between 1999 and 2004. Early morning well coughed out sputum specimens, broncho alveolar lavage, bronchial wash and endotracheal aspirate specimens were received from patients with clinical and radiological findings suggestive of pulmonary tuberculosis. From children and patients from whom expectorated sputum was not possible, gastric juice was collected. Biopsy and pus specimens were obtained from patients with lymphadenitis, abscesses or sinuses of chronic or recurrent nature. Entire early morning urine specimens were received from patients suspected of urinary tuberculosis. Sterile body fluids such as cerebrospinal fluid (CSF), blood, pleural fluid and others were collected from patients suspected of disseminated mycobacterial infections. All specimens were collected with aseptic precautions in sterile leak proof containers and transported to the laboratory.

The specimens were processed on the same day for microscopy and culture by standard procedures.[10] The specimens from ′unsterile′ sites such as sputum, urine and gastric juice were decontaminated by the Petroff′s method.[10] Sterile body fluids such as CSF, blood, pleural fluid and other fluids were processed without decontamination but after sterility check. Specimens in large volumes were centrifuged at 3000 rpm for 30 minutes and the deposit was used as inoculum. Biopsy specimens were ground with sterile precautions and processed. Smears were made and stained with auramine O and specimens were inoculated on to Lowenstein Jensen (LJ) media in duplicate and incubated at 370C. The cultures were examined everyday for one week and thereafter once a week for 6 weeks.

Smears were made from colonies on LJ medium and stained by Ziehl Neelsen stain. When colony morphology and smear morphology were suggestive of M. tuberculosis , they were further confirmed by Niacin test.[10] Those suspected to be NTM were subjected to a battery of biochemical tests for identification; these included nitrate reduction, aryl sulphatase (3 and 14 days), ability to grow in the presence of 5% NaCl, growth on MacConkey agar, susceptibility to polymixin B 300 units, pigment production, rate of growth, growth at 30 0C, 37 0C, and 45 0C, between 80 hydrolysis, potassium tellurite reduction, urease production, iron uptake and semi quantitative catalase.[11] Antimicrobial susceptibility testing for the rapidly growing NTM was carried out on Mueller Hinton agar by the disc diffusion Kirby Bauer method [12] to the following antibiotics: chloramphenicol (10 µg), erythromycin (15 µg), tetracycline (30 µg), vancomycin (30 µg), ciprofloxacin (5 µg), amikacin (30 µg), ofloxacin (5 µg), piperacillin (100 µg), cotrimoxazole (25 µg), rifampicin (5 µg), streptomycin (10 µg), gentamicin (10 µg), polymixin B 300, SSS (300 µg). For the slow growing NTM susceptibility to streptomycin, ethambutol, isoniazid and rifampicin were tested by the resistance ratio method in LJ medium.[13] The organisms were inoculated onto antibiotic containing media and the standard reference H37RV strain was included in each batch. The ratio of the MIC of the test strain to the MIC of the standard H37RV strain gave the resistance ratio based on which susceptibility profile was deduced.[13]

 ~ Results Top

Of the 32,084 specimens received for mycobacteria culture during the study period of six years 4473 (13.9%) grew acid fast bacilli (AFB). Four thousand three hundred (96.1%) of the acid fast bacilli were M. tuberculosis while 173 (3.9%) grew NTM from various clinical specimens with a presumptive diagnosis of mycobacterial infection. Of the 173 NTM 115 (66.5%) could be identified to the species level with the tests mentioned earlier, while the remaining could not be speciated.

Isolation of NTM from pulmonary and other specimens is shown in the [Table - 1]. Surgical specimens such as pus and biopsy specimens yielded largest number of NTMs followed by the respiratory specimens. M. chelonae (46%) and M. fortuitum (41%) accounted for the majority of the isolates [Figure - 1].

The median age of patients from whom NTM were isolated was 28 years in patients between 16 and 40 years, while in patients between 41 and 70 years it was 60 years. Sixty four (56%) were isolated from males and 51 (44%) were isolated from females. There was no seasonal variation in the isolation rates of NTM in all the six years.

Antibiotic susceptibility testing done for the rapid growers namely M. chelonae and M. fortuitum , showed highest susceptibility to amikacin (99.2%). Only one isolate of M. fortuitum and M. chelonae each were resistant to amikacin. Suceptibility to ofloxacin, ciprofloxacin and gentamicin in the case of M. fortuitum were 27%, 21% and 19.2% respectively but M. chelonae showed much lower susceptibility rates to these. Both the organisms exhibited very low susceptibility rates to the other antibiotics tested [Figure - 2]. Of the slow growers one strain of M. terrae was susceptible to all four drugs tested while 3 strains of M. szulgai and one strain of M. gordonae were susceptible to only ethambutol. All the others were resistant to all four drugs tested (streptomycin, ethambutol, isoniazid and rifampicin).

 ~ Discussion Top

NTM, also known as atypical mycobacteria, MOTT bacilli, environmental mycobacteria and opportunistic mycobacteria are saprophytes naturally distributed in soil, water and dust.[2] Nevertheless, these organisms have been reported to cause a variety of infections, more so in immunocompromised / immunosuppressed individuals and to a lesser extent in immunocompetant individuals. The incidence of tuberculosis has reduced in developed countries but infections due to NTM is on the rise,[17] while in developing countries like India, tuberculosis is still a major health problem; NTM are also reported frequently as causative agents of human infections.

Respiratory infections due to NTM are often associated with various conditions such as chronic obstructive pulmonary disease, cystic fibrosis of lung, bronchiectasis, emphysema of lung, previously treated pulmonary tuberculosis and lung cancer. Skin and soft tissue infections are frequently associated with post surgical wounds and injection abscesses. Other predisposing conditions include diabetes mellitus, leukemia, systemic lupus erythematosus, chronic alcoholism, AIDS and transplant patients.[2], [4]

M. kansasi, M scrofulaceum, M. fortuitum, Mycobacterium avium Scientific Name Search  complex , M xenopi and M. simiae have been reported to cause pulmonary infections.[2]

In our study, M. chelonae and M. fortuitum accounted for 67% of NTM isolated from respiratory specimens. M.fortuitum, M. chelonae and M. marinum have been commonly reported to cause skin and soft tissue infections, joint and bursae infections, wound infections and injection abscesses.[2] In this study M. chelonae and M. fortuitum were the predominant isolates from pus and biopsy specimens of patients with soft tissue infections and injection abscesses. Disseminated infections due to NTM have been widely reported in immunocompromised individuals especially in AIDS patients.[2]

In our study, only 7 (4%) isolates were from blood and CSF that could be considered as disseminated infections.

Since NTM are ubiquitous in nature and a possible laboratory contaminant, the isolation of these organisms from specimens should meet certain criteria to confirm their etiological significance such as, a) repeated isolation of the same organism from a patient, b) associated positive clinical and radiological evidence and c) histopathological confirmation. These parameters need to be considered while reporting NTM from clinical specimens. However, certain other parameters like a) collection of appropriate specimens directly from the lesion such as biopsies and BAL, b) isolation from sterile body fluids such as blood, CSF, pleural fluids, c) presence of any predisposing factors / underlying diseases and d) the immune status of the patient aids in assessing the etio pathogenesis of NTM when isolated.[2],[4] In our study NTM were repeatedly isolated from the sputum specimens of seven patients, biopsy and pus specimens from 15 patients, CSF specimens of two patients and blood cultures of two patients. Six were isolated from patients with AIDS and five were isolated from post transplant patients. Eight were isolated from sterile body fluids and 80 were from post surgical skin and soft tissue infections.

The management of NTM infections includes medical treatment with various antimicrobial agents based on susceptibility patterns and surgical treatment as in the case of lymphadenitis, skin or soft tissue infections. Since most of these organisms are resistant to commonly used antimicrobial agents, susceptibility testing becomes mandatory before instituting an effective therapy.

Karak et al from Kolkata, have reported a NTM prevalence of 17.4% from sputum specimens from patients with fibrocavitary pulmonary diseases,[3] this was comparatively higher than the reports of the other workers. Chakrabarthi et al from Chandigarh documented NTM isolation rate of 7.4% from various clinical specimens and M. fortuitum was the commonest isolate.[9] Paramasivam et al from Chennai, South India has reported 8.6% of NTM from sputum specimens of patients in BCG trial area. M. avium / intracellulare was the species most frequently isolated in their study.[14] Das et al reported isolation of 8.3% NTM from various clinical specimens from Delhi and Kasauli.[18] There has also been a report from our institution on the isolation of M. fortuitum from surgical specimens of patients with post operative wound infections, injection abscesses, skin and soft tissue infections.[15]

Although there are many reports from India, the exact disease burden of NTM infections still remains unclear in India. These infections are under diagnosed in many laboratories due to lack of facilities and expertise. These organisms can be often misidentified as Corynebacterium spp. or Nocardia spp.[16] Newer molecular methods such as gene probes, PCR and DNA fingerprinting may be better diagnostic tools.

In conclusion, regular documentation and reporting of these NTMs from clinical settings along with their sensitivity profiles is essential to be aware of the possible spectrum of diseases associated and preferred treatment options.

 ~ References Top

1.Wolinsky E. Non Tuberculous Mycobacteria and associated disease. Am Rev Respir Dis 1979; 119 :107-59.  Back to cited text no. 1  [PUBMED]  
2.Katoch V.M. Infections due to Non Tuberculous Mycobacteria (NTM). Indian J Med Res 2004; 120 :290-304.  Back to cited text no. 2    
3.Karak K, Bhattacharyya S, Majumdar S, De P.K. Pulmonary infections caused by mycobacteria other than M.tuberculosis in and around Calcutta. Indian J Pathol Microbiol 1996; 39 :131-4.  Back to cited text no. 3    
4.Wallace R J Jr, O'Brein R, Glassroth J, Raleigh J, Dutta A. Diagnosis and treatment of disease caused by Non Tuberculous Mycobacteria. Am Rev Respir Dis 1990; 142 :940-53.  Back to cited text no. 4    
5.O' Brian RJ, Geiter LJ, Snider DE. The epidemiology of Non Tuberculous Mycobacteria disease in the United States: Results from a national survey. Am Rev Respir Dis 1987; 135 :1007-14.  Back to cited text no. 5    
6.Tsukamura M, Kita N, Shimoide H, Arakawa H, Kuze A. Studies on epidemiology of Non Tuberculous Mycobacteriosis in Japan. Am Rev Respir Dis 1988; 137 :1280-4.   Back to cited text no. 6    
7.Matos ED, Santana MA, de Santana MC, Mamede P, de Lira Bezerra B, Parao ED, Filho CS, Lemos AC. Nontuberculous mycobacteria at a multiresistant tuberculosis reference centre in Bahia: Clinico-epidemiological aspects. Braz J Infect Dis 2004; 8 :296-304.  Back to cited text no. 7    
8.Choudhri S, Manfreda J, Wolfe J, Parker S, Long R. Clinical significance of nontuberculous mycobacteria isolates in a Canadian tertiary care center. Clin Infect Dis . 1995; 21 :128-33.   Back to cited text no. 8    
9.Chakrabarthi A, Sharma, Dubey M.L Isolation rates of different mycobacterial species from Chandigarh. Indian J Med Res 1990; 91 :111-4  Back to cited text no. 9    
10.Myer. R, Koshi. G. Manual of Diagnostic procedures in Medical Microbiology and Immunology / Serology (All India Press, Pondicherry) 1982:50-2.  Back to cited text no. 10    
11.Nolte FS, Metchock B. Mycobacterium . In : Chapter 31. Manuel of Clinical Microbiology 6th Edn. Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolken RH. Editors.Washington DC: ASM Press; 1995. p. 400-37.  Back to cited text no. 11    
12.Wallace RJ Jr, Davasio JR, Pankey GA. Disk diffusion testing of susceptibility of Mycobacterium fortuitum and Mycobacterium chelonei to antibacterial agents. Antimicrob Agents Chemother 1979; 16 :611-14.   Back to cited text no. 12    
13.Canetti G, Fox W, Khomenko A, Mahler HT, Menon NK, Mitchison DA, et al. Advances in techniques of testing mycobacterial drug sensitivity and the use of sensitivity tests in tuberculosis control program. Bull World Health Organ 1969; 41 :21-43.  Back to cited text no. 13    
14.Paramasivan C N, Govindan D, Prabhakar R, Somsundaran P R, Subbammal S, Tripathy S P. Species level identification of Non Tuberculous Mycobacteria from South Indian BCG trial area during 1981. Tubercle 1985; 66 :9-15.   Back to cited text no. 14    
15.Muthusami J C, Vyas F L, Mukundan U, Jesudasan M R, Govil S, Jesudasan S R. Mycobacterium fortuitum : An iatrogenic cause of soft tissue infection in surgery. ANZ J Surg 2004; 74 :662-6.   Back to cited text no. 15    
16.Garg P, Athmanathan S, Rao GN. Mycobacterium chelonae masquerading as Corynebacterium in a case of infectious keratits: a diagnostic dilemma. Cornea 1998; 17 :230-2.  Back to cited text no. 16    
17.Ferreira RM, Saad MH, Silva MG, Fonseca Lde S. Non-tuberculous mycobacteria I: one year clinical isolates identification in Tertiary Hospital Aids Reference Center, Rio de Janeiro, Brazil, in pre highly active antiretroviral therapy era. Mem Inst Oswaldo Cruz . 2002; 97 :725-9.  Back to cited text no. 17    
18.Das BK, Sharma VK, Bhanu LN, Saxena SN and Bhardwaj BK. Characterization of mycobacterial strains from clinical specimens. Indian J Pathol Micobiol 1982; 25 :19.  Back to cited text no. 18    


[Figure - 1], [Figure - 2]


[Table - 1]

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