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Year : 2002  |  Volume : 20  |  Issue : 4  |  Page : 230

Author's reply

Department of Microbiology, All India Institute of Hygiene and Public Health, Kolkata-700073, India

Correspondence Address:
Department of Microbiology, All India Institute of Hygiene and Public Health, Kolkata-700073, India

How to cite this article:
Kannan S. Author's reply. Indian J Med Microbiol 2002;20:230

How to cite this URL:
Kannan S. Author's reply. Indian J Med Microbiol [serial online] 2002 [cited 2020 Oct 28];20:230. Available from:

Dear Editor,
I would like to reply to the comments raised by Dr. Prema Bhat, Prashanthigram, A.P, regarding the article entitled “Isolation and Identification of Aeromonas from Patients with Acute Diarrhoea in Kolkata in India”, IJMM 2001;19(4):190-192.
Standard recommended procedures laid down in the Manual for Laboratory Investigations of acute enteric infections (WHO) were followed for recovering the recognized enteropathogens like Vibrio cholerae,  V.parahaemolyticus  ,  Salmonella More Details, Shigella, Rotavirus, Entamoeba, Giardia from the stool samples along with Aeromonas. Since the study aimed at the isolation and identification of Aeromonas the data regarding the other enteropathogens was not incorporated. Since faecal samples may contain varied microbial flora, and the presence of these microbes may inhibit the growth of Aeromonas an enrichment culture was made. Probably Dr.Bhat is not aware of the enrichment culture of Aeromonas. For further ready references, the following statement may be noted. Many authors have suggested to include an enrichment culture using APW before inoculating onto selective agar media while attempting the isolation of diarrhoeogenic Aeromonas from the stool samples. All the stool samples have been processed for the isolation of Aeromonas along with recognized enteric pathogens. Although all the parameters for the isolation of other enteric pathogens were followed, 64 Aeromonas strains isolated from the stool samples did not contain any established enteric pathogens. Regarding the AST, ciprofloxacin was tested, and 60% of the strains were sensitive and remaining were of intermediate sensitivity. The aim of incorporating the different antibiotics in this study was to characterize the different Aeromonas isolates. The incorporation of vancomycin in this study was done only to characterize the Aeromonas isolates. This study showed specific age related Aeromonas diarrhoea and found that children and the elderly patients were the most affected. We wish to clarify that there is no discrepancy between the number of isolates shown in results and in [Table:3]. This table highlights only the serotype prevalence in the isolated Aeromonas species. The number within brackets indicates the total Aeromonas species, 64 isolates. Aeromonas diarrhoea showed a clear seasonal association. It occurred in peak summer months and post monsoon seasons. This is only a portion of total work that we have carried out. We want to point out that the methodology for processing stool samples for isolation of diarrhoeogenic Aeromonas was already standardized by many investigators, and the same was followed by us in this study. 

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