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 ORIGINAL ARTICLE
Year : 2002  |  Volume : 20  |  Issue : 4  |  Page : 194-199

Evaluation of a new phage amplification technology for rapid diagnosis of tuberculosis


PD Hinduja National Hospital and Medical Research Center, Veer Savarkar Marg, Mahim, Mumbai - 400 016, India

Correspondence Address:
C Rodrigues
PD Hinduja National Hospital and Medical Research Center, Veer Savarkar Marg, Mahim, Mumbai - 400 016
India
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Source of Support: None, Conflict of Interest: None


PMID: 17657069

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PURPOSE: Rapid diagnosis of tuberculosis is essential to initiate timely and appropriate treatment to curb the spread of this potentially life threatening disease. The purpose of this study was to evaluate a phage amplification technology viz., FASTPlaque TB,™ for the diagnosis of tuberculosis. METHODS: We evaluated the clinical utility of this new assay by analyzing 50 respiratory and 40 non-respiratory specimens, using FASTPlaque TB™ kit (Biotec Laboratories, UK) and the performance was compared with TB Bactec 460 semi-automated liquid culture system and conventional LJ culture method. RESULTS: In case of respiratory specimens phage assay gave good specificity (100%) compared with TB Bactec whereas with respect to LJ method the sensitivity and specificity were 93.1% and 88.2% respectively. In case of non-respiratory specimens comparison of results obtained by phage assay showed sensitivity of 90.9% and specificity of 88.8% with respect to TB Bactec and 87.5% and 93.8% with respect to LJ method. CONCLUSIONS: We believe that this new low cost assay may have widespread applicability, especially in developing countries, due to its manual format and rapid reporting of results.






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