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 ORIGINAL ARTICLE
Year : 2002  |  Volume : 20  |  Issue : 1  |  Page : 33-36

Differentiation of pathogenic and saprophytic leptospires by polymerase chain reaction


1 Department of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar, Bareilly - 243 122, India
2 Department of Veterinary Microbiology, Veterinary College, Bangalore - 560 024, India
3 Institute of Animal Health and Veterinary Biologicals, Bangalore - 560 024, India

Correspondence Address:
R Noubade
Department of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar, Bareilly - 243 122
India
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Source of Support: None, Conflict of Interest: None


PMID: 17657021

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PURPOSE: To study the diferences between pathogenic and saprophytic leptospires. METHOD: A total of 275 samples were collected from different sources out of which 107 were subjected to PCR and bacteriological culturing. Two sets of primers were used for detection of leptospiral DNA and differentiation of pathogenic and saprophytic leptospires. Differentiation was also carried out by conventional methods. RESULTS: Twenty seven samples were found positive by PCR ut of which 26 were pathogenic and one was saprophytic. Culturing in EMJH medium yielded four isolates, of which isolates from sera were found to be pathogenic and isolate from water was found to be saprophytic. CONCLUSION: From the present study, it was concluded that PCR is simple, specific and rapid method for detection as well as differentiation of leptospires when compared to conventional methods.






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2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04