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 ~  Abstract
 ~  Materials and Me...
 ~  Results
 ~  Discussion
 ~  Acknowledgements
 ~  References

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Year : 2001  |  Volume : 19  |  Issue : 3  |  Page : 155-156

Penicillin resistant neisseria gonorrhoeae at Aurangabad

Microbiology Department, Govt. Medical College, Nanded, India

Correspondence Address:
Microbiology Department, Govt. Medical College, Nanded, India

 ~ Abstract 

A total of 101 male patients with signs and symptoms suggestive of gonococcal urethritis were studied and 60 showed growth of Neisseria gonorrhoeae on culture. All the isolates were tested for antimicrobial susceptibility. Four strains of Neisseria gonorrhoeae were resistant to penicillin. The resistant strains were tested for production of b-lactamase. The rapid iodometric method for detection of b-lactamase showed that two strains produced b-lactamase.

How to cite this article:
Bhatambare G S, Karyakarte R P. Penicillin resistant neisseria gonorrhoeae at Aurangabad. Indian J Med Microbiol 2001;19:155-6

How to cite this URL:
Bhatambare G S, Karyakarte R P. Penicillin resistant neisseria gonorrhoeae at Aurangabad. Indian J Med Microbiol [serial online] 2001 [cited 2021 Mar 7];19:155-6. Available from:

Gonorrhoea, a disease well documented from ancient times, continues to defy man's attempt to control it. The incidence of sexually transmitted disease is continuously increasing. Gonococcal infections and their complications are amongst the most frequent communicable diseases in many countries.[1] The present study was planned in view of increasing incidence and development of antibiotic resistance by  Neisseria More Details gonorrhoeae.

 ~ Materials and Methods Top

Sixty strains of  Neisseria More Details gonorrhoeae were isolated from 101 male patients with suspected gonococcal urethritis from January 1996 to December 1997, in Microbiology department of Government Medical College, Aurangabad.
Pus and urine samples were collected by standard procedure.[2] These were first examined by Gram stain[3] and then inoculated on Modified Thayer-Martin medium and Chocolate agar,[3] and incubated at 37 C in candle jar for 18 to 24 hours. The growth was recorded. Gram staining,[3] oxidase test,[3] sugar degradation test[4] and antimicrobial sensitivity tests[1],[5] were performed.
The antimicrobial susceptibility test[1],[5] using Kirby-Bauer disk diffusion method was carried out with ampicillin 10 g, cefotaxime 30 g, ciprofloxacin 3 g, co-trimoxazole 15 g, kanamycin 30 g, penicillin G 10 units and tetracycline 30 g.
The penicillin resistant isolates of  Neisseria More Details gonorrhoeae were tested for the production of b-lactamase. Rapid iodometric test was used for the detection of b-lactamase.[4]

 ~ Results Top

The four strains of  Neisseria More Details gonorrhoeae resistant to penicillin were tested for the production of b-lactamase. The rapid iodometric method for detection of beta-lactamase showed that two out of four strains produced b-lactamase. [Table - 1] shows results of antimicrobial susceptibility testing.

 ~ Discussion Top

The rapid iodometric method was performed on four penicillin resistant strains of  Neisseria More Details gonorrhoeae and it was found that two of them produced b-lactamase. This is an alarming sign as, there were no strains of  Neisseria More Details gonorrhoeae reported to be resistant to penicillin between1986 and 1987 in Aurangabad.6 Within ten years the incidence of penicillin resistance in  Neisseria More Details gonorrhoeae has risen to 6.66 % at Aurangabad. This emphasizes the importance of antimicrobial susceptibility testing in the course of treatment of gonorrhoea.
The b-lactamase producing  Neisseria More Details gonorrhoeae were detected for the first time in Aurangabad during this study. b-lactamase producing strains have been reported earlier in India from Madras (Chennai),[7] Bombay (Mumbai),[8] Trivandrum (Thiruvananthapuram),[9] Vishakhapatnam[10] and Chandigarh.[11]
[Table - 2] shows the results of studies carried out in India to detect b-lactamase producing strains of  Neisseria More Details gonorrhoeae

 ~ Acknowledgements Top

We thank the Dean, Government Medical College, Aurangabad, for providing us necessary facilities. 

 ~ References Top

1.World Health Organisation (Geneva). Neisseria gonorrhoeae and gonococcal infections. TRS 616. Geneva: WHO ; 1978.   Back to cited text no. 1    
2.Cheesbrough M, editor. Medical Laboratory Manual for Tropical Countries. Vol.2, Oxford: Butterworth-Heinemann Ltd; 1994.   Back to cited text no. 2    
3.Cruickshank R, Duguid JP, Marmion BP, Swain RHA, editors. Medical Microbiology. 12th ed. Vol. 2. NewYork: Churchill Livingstone; 1975.   Back to cited text no. 3    
4.Sonnenwirth AC, Javett L, editors. Gradwohl's clinical laboratory methods and diagnosis. 8th ed. Vol. 2. New Delhi: Mosby Co. ; 1982.   Back to cited text no. 4    
5.Roberts RB, editor. The gonococcus. New York : John Wiley and sons;1977.   Back to cited text no. 5    
6.Patel MN. Isolation of gonococcus and its drug sensitivity pattern (dissertation). Government Medical College, Aurangabad : Marathwada University ; November 1987.   Back to cited text no. 6    
7.Vijaylakshmi K, Gopalan KN, Gopalkrishnan B, Mohanram CC. The first case of b-lactamase strain of Neisseria gonorrhoeae from Madras. Ind J sex Trans Dis 1982; 3: 13.   Back to cited text no. 7    
8.Kulkarni MS, Shetty CR, Murti P. Antibiotic susceptibility of Neisseria gonorrhoeae. Ind J sex Trans Dis 1983; 4:1.   Back to cited text no. 8    
9.Moorthy PK, Thomas K, Sasidharan P, Papali C. Penicillinase (b-lactamase) producing Neisseriae from Kerala. Ind J Dermatol Venereol Leprol 1984; 50: 227.   Back to cited text no. 9    
10.Bhaskarrao K, Jayakar PA, Prasad ASV, Subharao M, Incidence of penicillin insensitive and b-lactamase producing strains of Neisseria gonorrhoeae in Vishakapatnam. J Ind Med Asso 1984;82:161.   Back to cited text no. 10    
11.Sharma M, Agarwal KC, Kumar B, Sharma SK. Penicillin resistant gonococci. Ind J Dermatol Venereol Leprol 1985; 51:22.   Back to cited text no. 11    
12.Malhotra VL, Natarajan R, Sing R, Agrawal DS, Screening Neisseria gonorrhoeae for the penicillinase production. Ind J Dermatol Venereol Leprol 1981;47:4.   Back to cited text no. 12    
13.Gohil AH, Kelkar SS, Antibiotic sensitivity of NeIsseria gonorrhoeae recorded in a Bombay Hospital. Ind J Med Res 1985;82:304.  Back to cited text no. 13    
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