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Year : 1999  |  Volume : 17  |  Issue : 4  |  Page : 190-193

Dot ELISA for antigen specific Circulating immune complex detection in Kala-azar

Department of Microbiology, King George's Medical College, Lucknow 226 003, U.P, India

Correspondence Address:
J Amita
Department of Microbiology, King George's Medical College, Lucknow 226 003, U.P
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Source of Support: None, Conflict of Interest: None

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ABSTRACT: Present study compares two simple ELISA tests for detection of antigen specific immune complexes (CIC) in the sera of patients of visceral leishmaniasis (VL). Their role in diagnosis of VL is evaluated. Sera from 50 positive controls, 50 negative controls (25 each endemic and nonendemic) and 40 patients (10 each of leprosy, malaria, amoebic hepatitis and tuberculosis) were tested. Immune complexes were precipitated with polyethylene glycol and their presence was tested by microwell and dot-ELISA. It was noted that both the ELISA were 100 percent sensitive, while dot ELISA was more specific compared to microwell ELISA (97.7 percent and 91.1 percent respectively with dot and microwell ELISA). The cross reactivity with dot-ELISA was 5.0 percent and with microwell ELISA, 20.0 percent. Hence dot-ELISA is preferred over microwell ELISA because it is simple to perform, needs less test time, is adaptable for field testing, needs least laboratory facilities and is more specific.

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2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04