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Year : 1999  |  Volume : 17  |  Issue : 4  |  Page : 187-189

Comparison of assessment of viability by normal mouse foot pad and PCR


Department of Experimental Leprosy, medicine, Microbiology & molecular Biology, Centre JALMA Institute for Leprosy (ICMR) Tajganj, Agra 282 001

Correspondence Address:
UD Gupta
Department of Experimental Leprosy, medicine, Microbiology & molecular Biology, Centre JALMA Institute for Leprosy (ICMR) Tajganj, Agra 282 001

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Source of Support: None, Conflict of Interest: None


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ABSTRACT: Comparision of viability assessment by mouse foot pad and PCR assay was studied in biopsy specimens from borderline lepromatous/lepromatous (BL/LL) cases. Biopsies were taken before the start of treatment and after treatment with various chemotherapeutic and immunotherapeutic regimens. These biopsies were processed and inoculated into mouse food pad (MFP) and also used for PCR assay by amplifying a part of 18kDa gene of M. leprae. It was observed that the trends of loss of viability by mouse foot pad correlated with reduction in PCR positivity. However, in 4-12.5 percent of leprosy cases PCR signals were found to persist even after MFP becoming negative at different time intervals. It appears that DNA targeting PCR may be suitable only for monitoring trends of therapeutic response. However, alternative methods are required to investigate the basis of persisting PCR signals.






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2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04