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Year : 1999  |  Volume : 17  |  Issue : 4  |  Page : 146-152

Molecular method in diagnosis and epidemiology of fungal infections

Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh

Correspondence Address:
A Chakrabarti
Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh

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Source of Support: None, Conflict of Interest: None

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ABSTRACT: Fungal infections have increased in incidence over the last two decades. However, most infections are diagnosed postmortem due to low sensitivity of conventional diagnostic methods. Recent progress in molecular biology may improve diagnostic capabilities. Several fungal specific probes as well as PCR based assays are being developed, and these may supplement, or perhaps even replace traditional methods for antemortem detection of candidemia, invasive aspergillosis and Pneumocystis carinii pneumonia. Molecular methods like multilocus enzymes electrophoresis, (MLE0 restriction enzyme analysis (REA), DNA hybridisation analysis, karyotyping and the use of polymerase chain reaction (PCR) for amplification of random polymorphic DNA fragments (RAPD) have also been used for understanding the epidemiology of fungal infections especially in nosocomial outbreaks. Recently, polymorphism of intergenic spacer region or rRNA repeat units, non transcribed spacer (NTS) region in rDNA and nucleotide sequence variation in the internal transcribed spacer (ITS 1 and 2) have been used for typing medically important fungi. Molecular methods also help in differentiation of relapse from reinfection in certain clinical situation.

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2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04