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| Year : 1999 | Volume
: 17
| Issue : 2 | Page : 72-73 |
Evaluation of elisa test using antigen 60 in the serodiagnosis of tuberculosis
J Kaur, DR Arora, R Sikka, KB Gupta
Department of Medical Microbiology, Pt. BDSPGIMS Rohtak, Haryana 124 001
Correspondence Address:
J Kaur
Department of Medical Microbiology, Pt. BDSPGIMS Rohtak, Haryana 124 001
 Source of Support: None, Conflict of Interest: None  | Check |
ABSTRACT: Serum samples from 100 tuberculosis patients and 50 controls were evaluated for the presence of IgA and IgG antibodies against antigen 60 by ELISA method using a commercial kit. The sensitivity and specificity of the test, considering cut-off values provided in the kit i.e. 350 for IgA and 225 units for IgG were determined. The sensitivity and specificity of the test for IgA were 43 percent and 90 percent respectively, whereas for IgG antibodies these values were 66 percent and 82 percent. When the cut off values were decreased to 200 units, the sensitivity of the test for IgA was 64 percent and for IgG 70 percent, and the specificity was 82 percent and 80 percent for IgA and IgG antibodies respectively.
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