|Year : 1999 | Volume
| Issue : 1 | Page : 26-28
Standardization of a nested polymerase chain reaction for cytomegalovirus.
GJ Finny, DJ Manayani, M Abraham, G Sridharan
Dept. of Clinical Virology, Christain Medical College and Hospital, Vellore 632 004, India
ABSTRACT: In this study we standardized a nPCR by using inner and outer primers to the morphological transforming region II of CMV and estimated its sensitivity in relation to plaque forming units (PFU) of the virus. Two sets of triplicate experiments were carried out to assess the sensitivity. The sensitive of the nPCR for CMV was estimated to be in the range of 0.01 to 0.001 PFU. This highly sensitive and specific nPCR for CMV can be used as a diagnostic test in post transplant immunosuppressed and HIV infected patients for the early detection of active CMV infection and therapeutic intervention in Indian settings.
Dept. of Clinical Virology, Christain Medical College and Hospital, Vellore 632 004
Source of Support: None, Conflict of Interest: None
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