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Year : 1999  |  Volume : 17  |  Issue : 1  |  Page : 10-13

Rapid detection methods for diagnosis of acute lower respiratory tract infection in young children due to respiratory syncytial virus.


Dept. of Microbiology, AIIMD, New Delhi, India

Correspondence Address:
RS Maitreyi
Dept. of Microbiology, AIIMD, New Delhi
India
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Source of Support: None, Conflict of Interest: None


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ABSTRACT: This study was designed to evaluate rapid diagnostic methods of detection of respiratory syncytial virus in children with acute lower respiratory tract infections (ALRTI). The methods used in this study included IFA staining on direct smears, centrifugation enhanced cultures combined with indirect immunofluorescence staining and RT-PCR. Nasopharyngeal aspirates (NPAs) were collected from 100 children under 5 years of age with ALRTI. RSV was detected in 9 by IFA staining on direct smears, by centrifugation enhanced cultures in 16 and by RT-PCR in 27 NPAs. Of 27 Strains detected by RT-PCR, 15 were typed as group A and 12 as group B by seminested PCR. RT-PCR was found to be a rapid method for detection and typing of RSV in clinical samples from children with ALRTI.






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2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04