|Year : 1999 | Volume
| Issue : 1 | Page : 10-13
Rapid detection methods for diagnosis of acute lower respiratory tract infection in young children due to respiratory syncytial virus.
RS Maitreyi, S Broor, SK Kabra, M Ghosh, L Dar, P Seth, AK Prasad
Dept. of Microbiology, AIIMD, New Delhi, India
ABSTRACT: This study was designed to evaluate rapid diagnostic methods of detection of respiratory syncytial virus in children with acute lower respiratory tract infections (ALRTI). The methods used in this study included IFA staining on direct smears, centrifugation enhanced cultures combined with indirect immunofluorescence staining and RT-PCR. Nasopharyngeal aspirates (NPAs) were collected from 100 children under 5 years of age with ALRTI. RSV was detected in 9 by IFA staining on direct smears, by centrifugation enhanced cultures in 16 and by RT-PCR in 27 NPAs. Of 27 Strains detected by RT-PCR, 15 were typed as group A and 12 as group B by seminested PCR. RT-PCR was found to be a rapid method for detection and typing of RSV in clinical samples from children with ALRTI.
Dept. of Microbiology, AIIMD, New Delhi
Source of Support: None, Conflict of Interest: None
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