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| Year : 1998 | Volume
: 16
| Issue : 4 | Page : 181-182 |
Comparison of staining methods of impression smears of antral biopsies for the rapid diagnosis of helicobacter pylori
M Anjana, M Srinivasa, P Mahalakshmi, V Savitha, R Kottoor, RS Macaden
Department of Microbiology St. John's Medical College and Hospital, Bangalore
Correspondence Address:
M Anjana
Department of Microbiology St. John's Medical College and Hospital, Bangalore
 Source of Support: None, Conflict of Interest: None  | Check |
ABSTRACT: It is recognised that Helicobacter pylori is an important gastric pathogen. The widely followed rapid diagnostic test is the urease test. However, not much attention is paid to staining. In the present study , a comparative evaluation of the staining method of Gram, two-step Gram, dilute carbol-fuchsin and Giemsa on impression smears obtained from 47 patients who attended the Gastroenterology department with symptoms of peptic ulcer diseases. The results showed that all these stains performed well when compared with the unrease test. However, the two-step Gram emerged as a better and more rapid stain with least background, closely followed by dilute carbol-fuchsin.
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