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Year : 1998  |  Volume : 16  |  Issue : 4  |  Page : 179-180

Preservation of streptococcus pneumoniae by stand desiccation

Department of Clinical Microbiology, C.M.C. Hospital, Vellore, Tamil Nadu 632 004

Correspondence Address:
KN Brahmadathan
Department of Clinical Microbiology, C.M.C. Hospital, Vellore, Tamil Nadu 632 004

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Source of Support: None, Conflict of Interest: None

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ABSTRACT: Capsular serogroups/types 1,5,7,19 and 23 of Streptococcus pneumoniae isolated from diverse invasive pneumoccal disease were preserved in multiple aliquots by sand desiccation and stored at room temperature (RT0 and +4 degree C. All stored cultures were subcultured at 0 day, 1 week and then at 1,4,16,24, 36 and 42 months to check for their viability. All cultures stored at +4 degree C were viable upto 4 months. Cultures preserved at RT became nonviable from 16 months onwards while those stored at +4 degree C were found viable upto 42 months. Our results show that sand desiccation is an ideal alternate method for preservation of S. pneumoniae when ultra low temperature and lyophilisation facilities are not easily available.

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2004 - Indian Journal of Medical Microbiology
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