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Year : 1998  |  Volume : 16  |  Issue : 2  |  Page : 66-68

Coagglutination test for rapid identification of adenovirus isolates

Division of Microbiology, Defence Research and Development Establishment Gwalior 474 002

Correspondence Address:
U Tuteja
Division of Microbiology, Defence Research and Development Establishment Gwalior 474 002

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Source of Support: None, Conflict of Interest: None

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ABSTRACT: Coagglutination test employing Staphylococcus aureus Cowan I strain sensitized with rabbit antiserum to hexon antigen of adenovirus type 5 was used for the rapid detection of adenoviruses from the acute respiratory diseases (ARD) of the children. Throat swab samples collected from 54 hospitalized cases with ARD were processed for virus isolation using HEp 2 cell line. Of the 31 CPE positive culture supermatants, 12 were positive for adenovirus by neutralization test (NT) and 9 among them were detected by coagglutination test. The 19 NT negative cases were also negative with the coagglutination test. Direct testing of 8 throat swap samples revealed 5 as positive in the coagglutination test and the same were confirmed to contain adenoviruses by NT.

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