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Year : 1998  |  Volume : 16  |  Issue : 1  |  Page : 23-25

Evaluation of two immunochromatographic assays in relation to `RAPID' Screening of HBsAG

Department of Clinical virology, Christian Medical College and Hospital, Vellore 632 004

Correspondence Address:
P Abraham
Department of Clinical virology, Christian Medical College and Hospital, Vellore 632 004

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Source of Support: None, Conflict of Interest: None

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ABSTRACT: Two immunochromatography based assays used for purposes of rapid screening for Hepatitis B surface antigen (HBsAG) were compared to our in-use third generation HBsAG ELISA, using a panel of 50 sera. Both assays yielded a similar sensitivity and specificity of 90 percent and 100 percent respectively. However, when both assays were evaluated independently using two sets of 400 consecutive sera, the Quickchaser and Virucheck assays yielded a sensitivity of 77 percent and 79 percent respectively and specificity of 99 percent and 97 percent respectively. the HBsAG positive sera missed by the Quickchaser assay were of significantly lower reactivity compared to those HBsAG containing sera that were positive by this assay and the ELISA, whereas for the Virucheck assay there was no such significant difference. The difference in evaluation results while using a smaller evaluation panel of 50 sera and large evaluation panel of 400 sera could be explained by a difference in the range of reactivities within both the panels and also a difference in panel size. This study reveals the need for a large panel of sera while evaluating tests to avoid overestimating the accuracy indices.

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2004 - Indian Journal of Medical Microbiology
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