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Year : 1995  |  Volume : 13  |  Issue : 3  |  Page : 141-144

Finger printing of salmonella typhimurium : comparison between traditional and molecular methods

Department of Microbiology, AIIMS, New Delhi

Correspondence Address:
I Alikhan
Department of Microbiology, AIIMS, New Delhi

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Source of Support: None, Conflict of Interest: None

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ABSTRACT: Traditional methods for characterisation of Salmonella typhimurium failed to clonally resolve the isolates from an infant and powder milk as well as from three different epidemiologically unrelated patients. All the strains had same biochemical reactions, antibiogram, serotype and were nontypable by phage typing. Herein, we report the successful application of whole cell protein analysis (WCPA), by SDS-PAGE, and plasmid profile to establish the clonal nature of these isolates, WCPA and plasmid analysis yielded profiles that indicated towards a common source for the origin of the epidemiologically related isolates and could clonally resolve these from epidemiologically unrelated isolates.

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2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04