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Year : 1993  |  Volume : 11  |  Issue : 3  |  Page : 191-196

Rapid Diagnosis Of Typhoid Fever By A New Elisa Test With Porin Antigens



Correspondence Address:
Jayasimha Rao


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Source of Support: None, Conflict of Interest: None


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A double antibody sandwich ELISA has been developed for porin antigens isolated from Salmonella, typhi using polyclonal antibodies raised in New Zealand white rabbits. Screening by the ELISA, of serum samples collected at a single time point from 91 patients with typhoid fever, 27 with Pyrexia of other causes; and 29 healthy controls, gave significantly higher porin values for typhoid patients compared to healthy controls (P<0.001) or other pyrexial cases (p<0.01). Application of this method to 14 paired blood samples collected during days 1-7 and 10-15 of fever showed no differences between first and second week samples in their identification as positive or negative for porins. Based on the data from healthy control and several Widal negative and positive pyrexial blood samples the following characteristics were computed for the present ELISA method. Sensitivity 92.9%, specificity 89.7%, accuracy 90.7%, positive predictive value 81.3% and negative predictive value 96.3%, respectively. The data suggests the usefulness of the ELISA in screening of patients for typhoid by serodiagnosis, as well as its probable utility in early serodiagnosis of the disease.






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2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04