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Year : 1993  |  Volume : 11  |  Issue : 3  |  Page : 170-177

Plasmid-Mediated Gentamicin Resistance In Methicillin Resistant Staph. Aureus In A Nursery Outbreak.


Correspondence Address:
V Kaul


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Gentamicin and methicillin resistant Staph. aureus (Gm - 76) was isolated from pus of a child with recurrent multiple abscesses. This strain spread to the nursery and infected eleven more patients. The Gm - 76 strain was investigated for the genetic determinant responsible for gentamicin resistance. Gentamicin resistant marker from Gm-76 strain was spontaneously lost which increased after treatment with ethidium bromide. This strain was lysogenic and mitomycin - C was effective phage inducer. A high frequency of transduction of gentamicin resistant marker was observed which decreased exponentially with UV - irradiated phage. An immediate phenotypic expression of gentamicin resistance was observed in transductants. As recombination deficient (rec-) strain was isolated after N-Methyl-N-nitro-N-nitrosoguanidine treatment form W 57 strains. The transduction frequency of the marker remained same when this strain was used as recipient. Gentmicin resistant plasmid was-isolated from gentamicin resistant parent strain. These results proved the plasmid nature of gentamicin resistance in Gm - 76 strain.






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2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04