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| Year : 1993 | Volume
: 11
| Issue : 2 | Page : 114-120 |
Studies Of Transfer Of Multiple Antibiotic Resistances In Escherichia Coli By Genetic Transformation
Jayasree Bhattacharyya, Sujata G Dastidar, A.N Chakrabarty
Correspondence Address:
Jayasree Bhattacharyya
 Source of Support: None, Conflict of Interest: None  | Check |
R plasmid carrying genetically defined strains of E.coli were screened for resistances to streptomycin, kanamycin, ampicillin, tetracycline and chloramphenicol. In a study of transfer of tetracycline resistance (Tc1) from multiple antibiotic resistant E.coli strains to different Tc-sensitive could be transferred singly and combindly with other antibiotic resistances at high frequencies following in situ genetic transformation system. Transformation was also achieved by applying a heat-pulse to CaCl2-treated recipient strains of E,coli. Presence of DNase (but not RNase) in transformations. Agarose gel electrophoresis showed the presence of R plasmids in the donors and their transformants.
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