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Year : 1993  |  Volume : 11  |  Issue : 2  |  Page : 114-120

Studies Of Transfer Of Multiple Antibiotic Resistances In Escherichia Coli By Genetic Transformation

Correspondence Address:
Jayasree Bhattacharyya

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Source of Support: None, Conflict of Interest: None

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R plasmid carrying genetically defined strains of E.coli were screened for resistances to streptomycin, kanamycin, ampicillin, tetracycline and chloramphenicol. In a study of transfer of tetracycline resistance (Tc1) from multiple antibiotic resistant E.coli strains to different Tc-sensitive could be transferred singly and combindly with other antibiotic resistances at high frequencies following in situ genetic transformation system. Transformation was also achieved by applying a heat-pulse to CaCl2-treated recipient strains of E,coli. Presence of DNase (but not RNase) in transformations. Agarose gel electrophoresis showed the presence of R plasmids in the donors and their transformants.


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2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04