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Year : 1992  |  Volume : 10  |  Issue : 2  |  Page : 114-117

Characterization Of R Plasmids In Salmonella Senftenberg Isolated During An Outbreak In Pediatric Ward

Correspondence Address:
Mohd.Najeeb Siddiqui

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Eighteen strains of Salmonella senftenberg, an uncommon serotype, were isolated from the stools of children attending the pediatrics clinics of JN Medical College Hospitals, AMU, Aligarh between January and May. All strains were multiple drug resistance. The commonest pattern was ACSTK (56.6%) followed by ACKT (22.3%). The MIC 90 against Ampicillin and chloramphenicol of these isolates was more than 500 ug/ml. The resistance could be cured following UV light or Acridine Orange treatment in some of these strains. The R plesmids did not have the property of auto-transfer when incubated with E. coli K 12, but could be mobilized by X factor with a mean frequency of 0.84 X 10-5, only ACKT could be transferred en bloc. Plasmid DNA was isolated from all the eighteen strains. All isolates had only one plasmid, which was determined to have a molecular weight of 48 kb.


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2004 - Indian Journal of Medical Microbiology
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