Indian Journal of Medical Microbiology IAMM  | About us |  Subscription |  e-Alerts  | Feedback |  Login   
  Print this page Email this page   Small font sizeDefault font sizeIncrease font size
 Home | Ahead of Print | Current Issue | Archives | Search | Instructions  
Users Online: 597 Official Publication of Indian Association of Medical Microbiologists 
 ~   Next article
 ~   Previous article
 ~   Table of Contents

 ~   Similar in PUBMED
 ~  Search Pubmed for
 ~  Search in Google Scholar for
 ~   Citation Manager
 ~   Access Statistics
 ~   Reader Comments
 ~   Email Alert *
 ~   Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded8    
    Comments [Add]    

Recommend this journal


Year : 1991  |  Volume : 9  |  Issue : 4  |  Page : 219-223

Detection Of Genomic Variation In Slow And Fast Growing Mycobacteria By Analysis Of restriction Fragment Length Polymorphism (RFLP) Of Ribosomal RNA Genes

Correspondence Address:
G. V Kanaujia

Login to access the Email id

Source of Support: None, Conflict of Interest: None

Rights and PermissionsRights and Permissions

Identifying and classifying pathogenic Mycobacteria is time consuming and remains a problem. We have earlier standardized techniques to study the variation in rRNA genes of Mycobacteria and have reported that restriction fragment analysis of rRNA genes of Mycobacteria could be useful for distinguishing some rapidly growing and slow growing Mycobacteria. In the present extended study, DNA was isolated from M.fortuitum, M.chelonei, M.avium, M.gordonae and M.kansasii. EAch DNA sample was cleaved with restriction endonclease EcoRI. After electrophoresis in gels, the DNA fragment containing rRNA gene sequences were transferred to nitrocellulose membranes and hybridized with -32p labeled Mycobacterial rRNA probe. The patterns of bands (the rDNA finger prints), which depended on Restriction Fragment Length Polymorphism (RFLP) of fragments containing rRNA genes, indicate that all these species could be distinguished from each other and patterns were found to be species specific. Results of this extended study show that this approach can be used for detection of genomic variation and rapid characterization of medically important Mycobacterial species.

[PDF Not available]*

Print this article     Email this article

2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04