Indian Journal of Medical Microbiology IAMM  | About us |  Subscription |  e-Alerts  | Feedback |  Login   
  Print this page Email this page   Small font sizeDefault font sizeIncrease font size
 Home | Ahead of Print | Current Issue | Archives | Search | Instructions  
Users Online: 199 Official Publication of Indian Association of Medical Microbiologists 
 ~   Next article
 ~   Previous article
 ~   Table of Contents

 ~   Similar in PUBMED
 ~  Search Pubmed for
 ~  Search in Google Scholar for
 ~   Citation Manager
 ~   Access Statistics
 ~   Reader Comments
 ~   Email Alert *
 ~   Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded4    
    Comments [Add]    

Recommend this journal


Year : 1990  |  Volume : 8  |  Issue : 2  |  Page : 40-43

Evaluation Of HSV-2 Bgl-II N Fragment

Correspondence Address:
J Kishore

Login to access the Email id

Source of Support: None, Conflict of Interest: None

Rights and PermissionsRights and Permissions

A recently developed sub-genomic DNA probe (7.2kb), HSV-2 Bgl-II N fragment cloned in PKC-7 plasmid has been evaluated in comparison to whole genomic HSV-2 DNA for specific detection of herpes simplex virus type-2 by filter hybridization. The probes were vector freed, nick-translated using 32pdCTP to a specific activity of 0.5-1 x 108 cpm/ug DNA. The whole gonomic HSV-2 DNA hybridized under stringent conditions with mock-infected cells and cells infected with other viruses as reveled by autoadiography. In contrast HSV-2 Bgl-II N fragment probe hybridized specifically only with HSV-2 infected vero cells and could detect 103 or more of such cells. Storage of the infected cells prior to hybridization at 4 C or –70 C for 24 hours did not alter the sensitivity or specificity of detection. Thus HSV-2 Bgl II N fragment can be used as rapid, specific and sensitive probe for the detection of herpes simplex virus type-2 infected cells.

[PDF Not available]*

Print this article     Email this article

2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04