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Year : 1988  |  Volume : 6  |  Issue : 4  |  Page : 337-341

Antifungal Susceptibility Testing In Otomycoses

Correspondence Address:
C Rodrigues

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Fungal aetiology was proved in 50 out of 63 clinically suspected cases of otomycosis. Fungi isolated were aspergillus niger (39), Aspergillus fumigatus (6), Penicillum spp (4) and Candida albicans (1). Antifungal susceptibility testing was performed by agar dilution method against amphotericin B. nystatin, clotrimazole, miconazole and 5-fluorocytosine (5 FC). The commonest isolate, Aspergillus niger was most susceptible to clotrimazole (mean MIC 2.7 mg/1) and in decreasing order to amphotericin B (3.16 mg/1), miconazole (3.66 mg/1), nystatin (4.2 mg/1) and FC (range of MIC 12.5 to > 50 mg/1). Clotrimazole showed satisfactory MIC values for other fungi as well. Mean MIC values against Aspergillus fumigatus were clotrimazole 2.21 mg/1, miconazole and nystatin 3.64 mg/1, amphotericin B, 3.90 mg/1, 5 Fc 12.5 - > 50 mg/1. mean MIC values against penicillium were clotrimazole 2.15 mg/1, miconazole 2.54 mg/1, 5 FC 3.51 mg/1, nystatin 3.90 mg/1, amphotericin B 4.69 mg/1. The methodology of antibiotic susceptibility testing of mycelial fungi is illustrated.

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2004 - Indian Journal of Medical Microbiology
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