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Year : 1988  |  Volume : 6  |  Issue : 4  |  Page : 251-258

Studies On The effect Of Rifampicin On Mycolic Acid Biosynthesis Of Mycobacteria : A Preliminary Report

Correspondence Address:
M. A Patil

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Source of Support: None, Conflict of Interest: None

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Mycolic acids are important class of mycobacterial lipids which form an essential component of cell-wall skeleton and could be important primary or secondary sites for drug action. With an aim to develop models for pathogenic mycobacteria, the effect of rifampicin on mycolic acid levels in two cultivable mycobacteria M. triviale and M. lufu has been investigated. The mycobacteria were grown in a incubator shaker in Sauton’s medium and were treated with varying concentrations of rifampicin in mid logarithmic phase. Samples were collected after 1, 2, 8, 24 and 48 hrs. for viability check and bacilli were harvested for mycolic acid analysis. The ‘bound lipid’ fractions comprising of mycolic acids were extracted and isolated from the cell wall structure M. triviale and M. lufu by alkaline methanolysis and were analysed by thin-layer chromatography using HP-TLC plates. The results showed that, rifampicin affected the mycolic acid levels at optimum concentrations. The higher concentrations, though, affected the viability, did not affect the mycolic acid levels indicating that both biosynthethic and catabolic processes are affected simultaneously resulting in poor or no degradation of mycolic acids present originally. These observations may have therapeutic implications and need to be further investigated.

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2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04