Indian Journal of Medical Microbiology IAMM  | About us |  Subscription |  e-Alerts  | Feedback |  Login   
  Print this page Email this page   Small font sizeDefault font sizeIncrease font size
 Home | Ahead of Print | Current Issue | Archives | Search | Instructions  
Users Online: 245 Official Publication of Indian Association of Medical Microbiologists 
 ~   Next article
 ~   Previous article
 ~   Table of Contents

 ~   Similar in PUBMED
 ~  Search Pubmed for
 ~  Search in Google Scholar for
 ~   Citation Manager
 ~   Access Statistics
 ~   Reader Comments
 ~   Email Alert *
 ~   Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded6    
    Comments [Add]    

Recommend this journal


Year : 1987  |  Volume : 5  |  Issue : 2  |  Page : 85-99

Glutaraldehyde Inactivated Pertussis Vaccine A Safe, Potent And Stable Vaccine

Correspondence Address:
R. K Gupta

Login to access the Email id

Source of Support: None, Conflict of Interest: None

Rights and PermissionsRights and Permissions

The whole cell pertussis vaccine was prepared using glutaraldehyde as an inactivating agent and evaluated with the routinely produced heat inactivated pertussis vaccine (HIPV) for potency, toxicity and stability. Five batches of each of glutaraldehyde inactivated pertussis vaccine (GIPV) and HIPV were prepared for each of B. pertussis strains 134 and 509. The potency of GIPV was very similar to that of HIPV showing no statistically significant differences (p>0.05). The GIPV had about 3 to 8 times less histamine sensitizing activity than HIPV. The mice injected with GIPV showed slightly higher weight gains than those injected with HIPV showing ‘lesser toxicity of GIPV in monse weight gain test. In the innocuity test also, the adsorbed diphtheria-pertussis-tetanus (DPT) vaccine containing GIPV was safer vaccine than the DPT vaccine containing HIPV. The stability of GIPV was similar to that of HIPV. Both the vaccines were stable in potency at 4-8C and 25C for three months while these lost about 60% of their potencies at 37C in 3 months. In this study it has been observed that glutaraldehyde under the prescribed conditions is a better inactivating agent than heat for preparation of a safe, potent and stable pertussis vaccine.

[PDF Not available]*

Print this article     Email this article

© 2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04